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Title: Best host for transduction of the camR gene Post by: karmahone on Apr 11, 2017 You are given three different strains of S. enterica for a transduction experiment. Strain 1 has a wild-type phenotype, strain 2 has a defect in oligosaccharide synthesis and strain 3 is defective in Histidine synthesis? Which of these strains make a suitable host for successful transduction of the camR (chloramphenicol resistance) gene using P22? Give reasons for your answer.
Title: Re: Best host for transduction of the camR gene Post by: bio_man on Apr 12, 2017 Strain 3 - histidine defective
Here's why: Chloramphenicol resistance can be found in strain 3. The structure of chromphenicol resembles histidine, therefore on deficient synthesis, the strain will not be attacked by chloramphenicol and will result in resistance. Title: Re: Best host for transduction of the camR gene Post by: bio_man on Apr 13, 2017 Can you tell me why you rejected the answer? A little feedback would go a long way lol
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