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Biology-Related Homework Help Genetics and Developmental Biology Topic started by: ilovelax on Mar 13, 2013



Title: What is the morphology/phenotypes of agrobacterium tumefaciens?
Post by: ilovelax on Mar 13, 2013
im isolating agrobacterium tumefaciens, i got a few isolates.However, when i compare to the one that i got a few years back, the morphology is quite different. So does anyone know the phenotype of this bacteria? i.e. the colour? shape? colony edges? and even better share me a picture of your agrobacterium colonies.


Title: What is the morphology/phenotypes of agrobacterium tumefaciens?
Post by: ilovelucy123 on Mar 13, 2013
Agrobacterium tumefaciens is a gram negative, motile, rod shaped bacterium which is non sporing, and is closely related to the N-fixing rhizobium bacteria which form root nodules on leguminous plants. The bacterium is surrounded by a small number of peritricious flagella. Virulent bacteria contain one or more large plasmids, one of which carries the genes for tumour induction and is known as the Ti (tumour inducing) plasmid. The Ti plasmid also contains the genes that determine the host range and the symptoms, which the infection will produce. Without this Ti plasmid, the bacterium is described as being non virulent and will not be able to cause disease on the plant.