Title: Expressing a human protein in bacteria Post by: spliceofslife on Jul 1, 2013 Lets say i wanted to express the human insulin protein in a bacteria, what part of this gene would i need to obtain?
Title: Re: Expressing a human protein in bacteria Post by: bio_man on Jul 1, 2013 The exons.
Introns are segments of DNA that are not needed to create a protein. Exons are segments of DNA that code for proteins. After mRNA is created by transcription of DNA, the introns are cut and removed by spliceosomes. The remaining exons are then spliced back together in the exact codon sequence needed to create the protein. |