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Title: After successfully incorporating into the genome of a fly, the construct DNA will: (Transgenic fly ... Post by: psychmajor74 on Aug 3, 2013 Part 1: Quiz
1. In this experiment, the reporter gene a.tells the experimenter exactly how much of the native protein was produced b.changes the color of the flies' eyes c.must be from the same type of organism as the organism into which it is being transferred d.is designed to be easily detected and reflect the activity of the native gene 2. After successfully incorporating into the genome of a fly, the construct DNA will a.change the functions of the native per gene b.cause the eyes to be white c.reflect transcriptional activity without interfering with functions d.alter the biological clock 3. Both fireflies and per-luc transgenic Drosophila a.can be seen glowing outdoors at dusk b.produce the enzyme luciferase c.use their glow to attract mates d.are experimentally produced 4. Which of the following DNA sequences is not found in the "construct DNA?" a.transposase b.luciferase c.period gene promoter d.P element Title: After successfully incorporating into the genome of a fly, the construct DNA will: (Transgenic fly ... Post by: bio_man on Aug 3, 2013 1. In this experiment, the reporter gene a.tells the experimenter exactly how much of the native protein was produced b.changes the color of the flies' eyes c.must be from the same type of organism as the organism into which it is being transferred d.is designed to be easily detected and reflect the activity of the native gene I'm gearing more towards D, but A is also a possibility. 2. After successfully incorporating into the genome of a fly, the construct DNA will a.change the functions of the native per gene b.cause the eyes to be white c.reflect transcriptional activity without interfering with functions d.alter the biological clock This piece of information should help you answer this question: A DNA construct is an artificially constructed segment of nucleic acid that is going to be "transplanted" into a target tissue or cell. It often contains a DNA insert, which contains the gene sequence encoding a protein of interest, that has been subcloned into a vector, which contains bacterial resistance genes for growth in bacteria, and promoters for expression in the organism. A DNA construct may express wildtype protein, prevent the expression of certain genes by expressing competitors or inhibitors, or express mutant proteins, such as deletion mutations or missense mutations. A DNA construct is often used in molecular biology to analyze macromolecules such as proteins or RNA in more detail. Title: After successfully incorporating into the genome of a fly, the construct DNA will: (Transgenic fly ... Post by: psychmajor74 on Aug 3, 2013 Thanks a lot for your help. I appreciate it :D
Title: After successfully incorporating into the genome of a fly, the construct DNA will: (Transgenic fly ... Post by: barry on Aug 3, 2013 3. Both fireflies and per-luc transgenic Drosophila a.can be seen glowing outdoors at dusk b.produce the enzyme luciferase c.use their glow to attract mates d.are experimentally produced 4. Which of the following DNA sequences is not found in the "construct DNA?" a.transposase b.luciferase c.period gene promoter d.P element Did you make an guesses here? Title: After successfully incorporating into the genome of a fly, the construct DNA will: (Transgenic fly ... Post by: bio_man on Aug 3, 2013 You're welcome.
Title: After successfully incorporating into the genome of a fly, the construct DNA will: (Transgenic fly ... Post by: psychmajor74 on Aug 3, 2013 I just completed the test and you will find the answers as followed. Some of my answers were wrong, but I provided the CORRECT answer. I hope this helps.....this was pretty difficult!
Make Transgenic Flies Part 1: Prepare DNA to be incorporated into the fly genome Quiz Answers: (Click to review) 1. Correct! Your answer: D. 2. Correct! Your answer: C. 3. Correct! Your answer: B. 4. Correct! Your answer: A. Part 2: Prepare fly embryos Quiz Answers: (Click to review) 1. Correct! Your answer: D. 2. Correct! Your answer: C. Part 3: Inject fly embryos with DNA Quiz Answers: (Click to review) 1. Correct! Your answer: C. 2. Correct! Your answer: B. Part 4: Breed flies Quiz Answers: (Click to review) 1. Incorrect. Your answer: C. The correct answer is D. 2. Correct! Your answer: B. Part 5: Select transgenic progeny Good, you found some red-eyed flies. No quiz for this part. Part 6: Examine light output from transgenic adults Quiz Answers: (Click to review) 1. Correct! Your answer: C. 2. Incorrect. Your answer: B. The correct answer is A. Use Transgenic Flies Experiment 1: Measure per-luc gene expression while keeping lights off Your Hypothesis: Daily light-emission oscillations will not be detected from flies kept in constant darkness. Conclusion: Your Hypothesis is incorrect. Quiz Answers: (Click to review) 1. Incorrect. Your answer: A. The correct answer is B. 2. Incorrect. Your answer: B. The correct answer is A. 3. Incorrect. Your answer: A. The correct answer is B. Experiment 2: Measure per-luc gene expression while changing light on-off times Your Hypothesis: Changing the light-dark cycles that flies are kept in resets the flies' internal clock, resulting in a shift of the peaks and troughs in the daily light-emission oscillations. Conclusion: Your Hypothesis is correct! Quiz Answers: (Click to review) 1. Correct! Your answer: B. 2. Correct! Your answer: A. 3. Incorrect. Your answer: B. The correct answer is A. Experiment 3: Examine different fly body parts for per-luc expression Your Hypothesis: Oscillating per gene expression (measured as light emissions) is found only in the Drosophila's wings. Conclusion: Your Hypothesis is incorrect. Quiz Answers: (Click to review) 1. Correct! Your answer: D. 2. Incorrect. Your answer: B. The correct answer is D. |