Title: Palindromic DNA sequence Post by: Valentine10 on Nov 4, 2010 I just can't seem to get this question right at all!
'The restriction endonuclease Sac I recognises a 6-nucleotide palindromic DNA sequence whose first three bases are 5 prime - GAG - 3 prime. Sac I cuts to give a 4 - nucleotide 3 prime overhang that is centred on the middle of the sequence. What is the base sequence of the 4 - nucleotide overhang when read towards the free end of the chain? 3 Prime - TGCA - 5 Prime 3 Prime - AGGA - 5 Prime 5 Prime - AGGA - 3 Prime 5 Prime - TCGA - 3 Prime 5 Prime - AGCT - 3 Prime Any help would be greatly appreciated! :) Title: Re: Palindromic DNA sequence Post by: bio_man on Nov 4, 2010 Here's an example of a palindromic sequence:
5'- G A A T T C -3' 3'- C T T A A G -5' I think it's: 3 Prime - TGCA - 5 Prime ... because 3 Prime - ACGT - 5 Prime And I chose this instead of 5 Prime - AGCT - 3 Prime because the question states 5 to 3 and the opposite of this would be 3 to 5. Title: Re: Palindromic DNA sequence Post by: Valentine10 on Nov 5, 2010 Thank you! :)
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