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Title: Shotgun Sequencing Post by: adrii01 on Nov 17, 2013 In the shotgun approach to whole-genome sequencing (shotgun sequencing), random DNA fragments of a chromosome are sequenced. The fragment sequences are then assembled into a continuous sequence that represents the DNA of the entire chromosome.
What are the steps in the shotgun approach to whole-genome sequencing? Drag the labels to their appropriate locations on the flowchart. Only some labels will be used. Title: Re: Shotgun Sequencing Post by: padre on Nov 17, 2013 1) multiple copies of the same chromosome are prepared
2) Chromosome copies are broken into 1-kb fragments 3) 1 kb fragments are cloned into plasmids 4) the plasmids are sequenced 5) A computer combines the fragment sequences There is no use for RNA (1-kb fragments are transcribed into RNA) in the Sanger method = full genome sequencing i.e. the shotgun approach. Title: Re: Shotgun Sequencing Post by: queenhoa on Nov 17, 2013 Content hidden
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