× Didn't find what you were looking for? Ask a question
Top Posters
Since Sunday
5
o
5
4
m
4
b
4
x
4
a
4
l
4
t
4
S
4
m
3
s
3
Are you an expert?
Quickly gain a reputation by helping other students with their questions. When students see your nickname, they'll immediately associate your answer with credibility and expertise. Also, earn credits for sharing your knowledge and redeem them for rewards.
I am good at  
Delay Artefacts In Nerve Conduction Velocity
Delay Artefacts In Nerve Conduction Velocity
I was shown a demonstration on estimating peripheral nerve conduction velocity in a healthy subject and have been asked about to look up about delay artefacts which limits the accuracy of the values. There is apparently two of these but I have not been successful in finding out either of these. I am thankful for any help.
The demonstration is similar to this:

mustel
Upper-Year Courses   Archer502   497   Asked 6 years ago
(surface plasmon resonance) which method are helpful for reduce negative curve?
(surface plasmon resonance) which method are helpful for reduce negative curve?
1X tbs w/ sample
(1X TBS buffer : 90% DW+10% 10X TBS)

<1>
sample 100ul(diluted at DW until 1000X)
DW 800ul
10X TBS 100ul

it's my co-worker's opinion. he said "sample in DW, then when you dilute in 1X TBS buffer, it's final conc. will 0.9X. negative curve occurs by conc. mismatch, and we need to 1X TBS, so this metho
Upper-Year Courses   Koreanraichu   761   Asked 7 years ago
During chlorophyll biosynthesis, which wavelength of light, if at all, would produce the optimum ...
During chlorophyll biosynthesis, which wavelength of light, if at all, would produce the optimum ...
I am performing an experiment of chlorophyll biosynthesis, so had a question about whether during the reduction of the D ring by NADPH in chlorophyll biosynthesis, is the process affected by different wavelengths of light. It will also be very helpful if someone could suggest me a site to get data on the amount of chlorophyll in a mung bean and barley plants
Upper-Year Courses   jashdhill   1031   Asked 7 years ago
-20 microbial glycerol stocks
-20 microbial glycerol stocks
hello,

I am keeping microbial stocks in 25% glycerol at -20 (-80 not available) for a few months. but I am noticing that the stocks are remaining liquid. will this affect the viability of the cells ? if so what can I do without repeating the whole process again. thank you
Upper-Year Courses   tahah   1318   Asked 7 years ago
Micronucleus testing, stain too dark?
Micronucleus testing, stain too dark?
Hello there!  First time posting.  Slight Smile

In any case, we did a micronucleus test on rat bone marrow in class this past week.  I followed the directions to a T, and timed my staining to the second, with my slide in the Wright stain for exactly 2 minutes.  Still, most of my erythrocytes were too dark to see anything, and I ended up just searching for paler
Upper-Year Courses   Wifey2myWifey   824   Asked 7 years ago
Why heat can not reverse my formaldehyde cross-linking product
Why heat can not reverse my formaldehyde cross-linking product
I was trying to pull down some associated proteins with my bait protein by formaldehyde cross-linking. my bait protein is 3XFLAG-tagged. So my final step is to elute my proteins out by Low pH glycine. Then, the eluted protein was split into two samples. One is kept in ice after adding 2X Laemmli sample buffer, and the other is heated at 90 degree for 1 hour with sample buffer. Surpr
Upper-Year Courses   wuchenggang   1508   Asked 7 years ago
Testing Cancer Cells ?
Testing Cancer Cells ?
Hi,

I would like to test cancer cells. Practical Tests.
I can imagine to run some tests from cancer cells of a mouse.
Isolating them in a petri dish and testing different substances and reactions on them.

Are there any video tutorials ?
A step by step guide ?
How to get started ?


Thank you so much for any little help
Upper-Year Courses   remoz   901   Asked 8 years ago
How does the electronegativity of a metal affect the activity of catalase?
How does the electronegativity of a metal affect the activity of catalase?
   Without any further ado, I have a problem concerning my lab experiment. The research question about the topic of enzymes with which I came up (the shortened version of it is the title) is the one that I found the most amusing and interesting. So I went for it. I predicted the activity to go down as the electronegativity of the metal that is in the cation of a salt increased, thus,
Upper-Year Courses   Malefactor   1077   Asked 8 years ago
What does it mean by induced/uninduced cultures?
What does it mean by induced/uninduced cultures?
When growing recombinant proteins I used a uninduced control culture and a auto induction medium in my production culture.

what does it mean by induced/uninduced
Upper-Year Courses   GCUQMU   1299   Asked 8 years ago
Difficulty to come to a cunclusion from my SDS-PAGE gel electrophoresis results.
Difficulty to come to a cunclusion from my SDS-PAGE gel electrophoresis results.
In class I did a sds-page electrophoresis of e.coli expressed in four different sugars; Frucose, Arabinose, Glucose and Galactose, all in a concentration of 30 µl. When looking at my sds-page result, the four different samples seem to express the same proteins. The only difference that is noticible is the transparency of the blue color, but that's it.

I'm wonde
Upper-Year Courses   Woahscience   2604   Asked 8 years ago
please write a full discussion in this H Nmr and explain each signal
please write a full discussion in this H Nmr and explain each signal
I need help on H NMR ,i have to writea discussion on this specturm,I know the structure of product is D- glucose, but i could not write a discussion on it. Please explain everythingabout theses spectrum and how each signal related to the D- Glucose.

Please help me this is for my lab report.

Please explain everything.
I have to write a paper on it but I co
Upper-Year Courses   Amin.noorian   1229   Asked 8 years ago
How to calculate the stock material in PCR?
How to calculate the stock material in PCR?
Hi! I have a problem with filling this PCR table. Can anyone help me, please?Slight Smile
Upper-Year Courses   notimp   3431   Asked 8 years ago
Expression data - how to deal with it?
Expression data - how to deal with it?
Hello dear forum
I am working currently with the expression analysis of various genes by Affymetrix microarray chips. Here I come to terms with which I unfortunately can not do much.

In one illustration the absolute expression is specified (unitless values between 0 and 2000), in the description they say, "normalization GCOS: TGT (100)".  GCOS is proba
Upper-Year Courses   Jaccy   4546   Asked 8 years ago
Design an experiment outlining your techniques that will analyze the temporal expression pattern of
Design an experiment outlining your techniques that will analyze the temporal expression pattern of
Design an experiment outlining your techniques that will analyze the temporal expression pattern of a gene (gene X - mRNA expression) and its protein localization during gastrulation in three different organisms.
Please state the organisms you are choosing, the time in which you will perform the experiment.
Please also state the molecular techniques you will use to follow
Upper-Year Courses   gkor29   3127   Asked 9 years ago
TTEST calculations help!
TTEST calculations help!
Hi there, I had generated a knockdown cell pool using shRNA against my target gene and control. i then subject these cells to the same conditions (DNA damage, etc).

I run experiments in triplicate usually, and then generate them independently 3 times.

i have some really basic questions, if you could help that would be great.

1. when i calculated stan
Upper-Year Courses   mm123an   2364   Asked 9 years ago
Formulation of a questionnaire based on Birth Order and Coping with Stress
Formulation of a questionnaire based on Birth Order and Coping with Stress
Pleasant Good Day to All,
I am doing my Thesis on the topic Birth Order and Coping. I am in the process of trying to put a questionnaire together that helps match my independent and dependant variables: Ordinal Position, Archetypical Profile, Dethronement of First Born Coping Strategies, Resilience/Vulnerability and environment. Can anyone suggest questions that they think can
Upper-Year Courses   Rhyanbeau   821   Asked 9 years ago
Calculating GFR
Calculating GFR
I need to calculate GFR using creatinine clearance and MDRD equation. All the data I have is two absorbances for a standard (start = .321) (stop = .358) and two absorbances for urine (start = .288) (stop = .456).  The urine volume was 230 mL.  The absorbances were taken at A500.

Help would be GREATLY APPRECIATED!

Mostly I just need help in calculatin
Upper-Year Courses   guitargirl0812   1045   Asked 9 years ago
Can pGEM-T Easy Vector JM109 cells take up multiple copies of the Plasmid?
Can pGEM-T Easy Vector JM109 cells take up multiple copies of the Plasmid?
I am using JM109 cells and the pGEM-T easy vector system II to clone a mixed microbial community for sequencing and I need to know whether a sinle copy or multiple copies can be taken up by each competent cell because this will impact on my ability to sequence from purified plasmid from 1 white cell.
Upper-Year Courses   !JRon@n   868   Asked 10 years ago
what would cause the different migration rate between each wells in agarose gel electrophoresis?
what would cause the different migration rate between each wells in agarose gel electrophoresis?
I have been having this trouble. DNA sample travels at different rates between different wells but I know they have the same size. The wells toward the middle of the gel always travel slower and the bands across the gel look like a rainbow shape. It happen all the times, so I think there is something wrong since it has been consistently giving this "rainbow migration pattern".
Upper-Year Courses   flowe12   1450   Asked 11 years ago
Molecular biology of the cell study guides
Molecular biology of the cell study guides
Does anyone know where i could find study guides to Molecular biology of the cell by alberts, et al?
Upper-Year Courses   forsheyc1   2354   Asked 11 years ago
Help with Exercise 3 Neurophysiology of Nerve Impulses please?
Help with Exercise 3 Neurophysiology of Nerve Impulses please?
Activity 2 Q 3. Identify which of the stimulus modalities induced the largest amplitude receptor potential in the Pacinian corpuscle. How well did the results compare with your prediction?

A2 Q4. Identify which of the stimulus modalities induced the largest-amplitude recptor potential in the olfactory receptors. How well the the results compare with you prediction?
Upper-Year Courses   HollyEV   7033   Asked 12 years ago
Human Anatomy Exercises Post 6
Human Anatomy Exercises Post 6
I am posting some of my completed exercises from the lab manual Human Anatomy Laboratory Manual with Cat Dissections (6th ed.) by Elaine Marieb and Susan J. Mitchell. I will warn you ahead of time some of the scans are not straight, there might be double copies of a page in the scanned exercise but oh well at least the answers are right. 
Upper-Year Courses   zanydelight69   1367   Asked 12 years ago
In the experiment, explain what happened to active force as the ‘voltage’ increased? As it
In the experiment, explain what happened to active force as the ‘voltage’ increased? As it
Hello there!
  I just completed activities 6-8 in exercise 2 of the Phsyio Ex 8.0 book, and my professor wants me to to be able to answer some questions related to the experiments.  I answered most of them, but I'm not entirely sure about these (I've bolded the questions and italicized any of the answers I've already given):

[
Upper-Year Courses   Kate   7045   Asked 12 years ago
PhysioEx 18B Solutions
PhysioEx 18B Solutions
Hey there, does anyone have the solutions to PhysioEx 18B Neurophysiology of Nerve Impulses? I've done the questions but I wanna check them over before I hand it in so I can make sure I do well on it. It's from the A&P lab manual. Thanks
Upper-Year Courses   ErikIsAHuman   2585   Asked 12 years ago
PhysioEx 8.0 exercise 4
PhysioEx 8.0 exercise 4
Looking for key to exercise 4 activity 5-8.  Must grade papers for instructor tonight, thank you for any help possible.
Upper-Year Courses   tigger too!   1938   Asked 13 years ago
What is the relationship between nerve size and conduction velocity?
What is the relationship between nerve size and conduction velocity?
I have finished the lab but am stuck on the last 3 questions in the activity 8.

5. What is the relationship between nerve size and conduction velocity?  What are the physiological reasons for this relationship?
I know that the relationship is the larger the nerve the faster the velocity, but I need help with the physiological r
Upper-Year Courses   havoc-covah   2522   Asked 13 years ago
Describe your four tracings and explain what has happened in each of them.
Describe your four tracings and explain what has happened in each of them.
I am stuck on just a few questions and wondered if anyone could help me out.

Activity 3 question: An individual muscle fiber follows the all-or-none principle—it will either contract 100% or not at all.  Does the muscle we are working with exhibit the all-or-none principle?  Why or why not?

Activity 9 question:  Describe your four tracings and ex
Upper-Year Courses   sunshine   1592   Asked 13 years ago
Explore
Post your homework questions and get free online help from our incredible volunteers
  950 People Browsing
 114 Signed Up Today
Gallery
  
 158
  
 1646
  
 1756