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What is the minimum bactericidal concentration of each antibiotic?
The broth dilution test can be used to determine the effectiveness of an antibiotic. In this test, serial dilutions of the antibiotic were set up in the wells of a microtiter plate. Equal amounts of broth culture of Staph aureus were added to each well. After incubation, the wells were examined for bacterial growth. Wells with no growth were subcultured in nutrient broth without the
The average adult needs about 2000 calories per day. There are a little less than 4 dietary calories (kCal) in a gram of sucrose (table sugar). One mole of sucrose weighs 342.3 grams. A mole of anything is 6.02x10^23 molecules. Dietary fats (usually 18 to 20 carbons) contain approximately 8 dietary calories per gram. Assuming all of the food that people eat goes to making ATP,
A directional (forced) clonning is planned using the restriction enzymes Pst1 and Sph1 to generate incompatible termini in the vector pUC18. Note that enzymes have different optimum buffer requirements and must be digested sequentially rather than simultaneously. The plasmid is first digested with Sph1, purified on a column, eluted and resuspended in Pst1 buffer and digested with Ps
A vector was prepared by a double restriction digest (EcoR1 + BamH1) to generate different ends for zero background colonies. Say you preformed a parallel experiment using the different enzyme pair Sma1 and Hae111 to prepare the vector, which was then ligated to fragments with Sma1/Hae111 ends. What result would be obtained when the transformation plates were examined? 4marks... i h
Agonist antibodies are able to activate a receptor-mediated response in target cells via their inter
Agonist antibodies are able to activate a receptor-mediated response in target cells via their interaction with that receptor. Discuss the advantages and disadvantages of an agonist antibody as a therapeutic agent for immune regulation.
Of the following electron carriers of the electron transport system, which transfers protons in addi
Of the following electron carriers of the electron transport system, which transfers protons in addition to electrons? A) cytochrome a B) cytochrome b C) coenzyme Q D) iron-sulfur proteins E) iron-copper proteins
Key points in this Hematopoietic Stem Cell article?
I was pointed to this article to answer some questions. Now I don't expect to have all these questions answered, I just need some guidance on some key points because I am very new to reading these research papers. This is the first paper I've read and I urgently need help. Anything would be great appreciated. Thanks!
Explain how each of the following avoids being killed by phagocytes. a.Streptoc
Explain how each of the following avoids being killed by phagocytes. a. Streptococcus pneumoniae b. Mycobacterium tuberculosis c. Streptococcus pyogenes d. Shigella dysenteriae
A patient with a heart pacemaker received antibiotic therapy for streptococcal b
A patient with a heart pacemaker received antibiotic therapy for streptococcal bacteremia (bacteria in the blood). One month later, he was treated for recurrence of the bacteremia. When he returned 6 weeks later, again with bacteremia, the physician recommended replacing the pacemaker. Why did this cure his condition?
Assume that you are responsible for decontaminating materials in a large hospita
Assume that you are responsible for decontaminating materials in a large hospital. How would you sterilize each of the following? Briefly justify your answers. a. A mattress used by a patient with bubonic plague. b. Intravenous glucose-saline solutions. c. Used disposable syringes. d. Tissues taken from patients.
Pseudomonas syringae is found naturally in the soil. Sold as Snomax, it is used
Pseudomonas syringae is found naturally in the soil. Sold as Snomax, it is used to make snow at ski resorts. The same bacterium with a gene deletion (Ice-minus) is used to prevent ice formation on plants. Should Snomax and Ice-minus be considered modified organisms and subject to precautions of releasing genetically modified organisms? Explain why or why not.
Use the information given in Table 10.3 to answer questions (a) and (b). Note t
Use the information given in Table 10.3 to answer questions (a) and (b). Note to instructors: A=Pseudomonas; B=Escherichia; C=Staphylococcus; D=Neisseria; E=Alcaligenes. a. Which organisms are most closely related? On what did you base your answer? b. DNA from which organisms will probably hybridize?
A patient consulted a physician for symptoms that included a swollen toe, a red
A patient consulted a physician for symptoms that included a swollen toe, a red streak along his ankle, and enlarged lymph nodes in his groin. Explain the cause of these symptoms.
A teenaged boy knew an over-the-counter cortisone preparation would decrease the
A teenaged boy knew an over-the-counter cortisone preparation would decrease the swelling and redness of insect bites and poison ivy, so he used it to decrease the swelling and redness of pimples. Why, in 24 hours, were his pimples more inflamed?
On June 1, a 32-year-old hiker was bitten by a tick. After 1 week, he noticed an
On June 1, a 32-year-old hiker was bitten by a tick. After 1 week, he noticed an erythrematous ring at the location of the bite. Four weeks later, a physician found a large, macular, centrifugally spreading ring. During the next month, it expanded to 35 cm and faded. Over the next 2.5 years, the man experienced recurrent inflammation of a knee. Identify the periods of incubatio
Nearly 70% of the patients seen in STI clinics are men. a.Offer a reason men ar
Nearly 70% of the patients seen in STI clinics are men. a. Offer a reason men are more likely to seek treatment than women. b. Why is it important that women seek treatment for STIs?
How can you avoid contracting the following diseases?
How can you avoid contracting the following diseases? a. Trichinellosis b. Hydatidosis c. Tapeworm infestations d. Staphylococcal food poisoning e. Traveler's diarrhea f. Giardiasis
What is the effect of discharging primary-treated sewage on the BOD and dissolve
What is the effect of discharging primary-treated sewage on the BOD and dissolved O2 (DO) of the receiving body of water? What is the effect of secondary-treated sewage on the BOD and DO?
A newspaper headline reported "Algal Bloom Kills Fish." a. What actually caused fish death, given that the algae were not toxic? b. What could have caused the algal bloom?
Most of the world's population relies on wheat for food. Research is being condu
Most of the world's population relies on wheat for food. Research is being conducted to produce wheat with a higher protein content. Design a biotechnological approach to improving the amino acid content of wheat.
Worldwide, which disease is the most serious public health threat: malaria, Chag
Worldwide, which disease is the most serious public health threat: malaria, Chagas’ disease, or brucellosis? Explain why, and provide a plan to reduce the incidence of the disease.
