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Find the size of amplicon if we use the following PCR-primers for human GLUT1
Find the size of amplicon if we use the following PCR-primers for human GLUT1
Hello,

I have an exericise to solve at home:


What is the size of GLUT1 amplicon if we use the following PCR-primers for human
GLUT1? Please remember that we used human GLUT1 cDNA as a template.

GLUT1 sense: 5´AACTCTTCAGCCAGGGTCCAC 3´

GLUT1 antisense: 5´CACAGTGAAGATGATGAAGAC 3´


I tried to solve it myself but I d
Genetics and Developmental Biology   mashu   451   Asked 8 years ago
need help please
need help please
1- Baur and Shull crossed broad-leaved females of the plant species Lychnis alba to narrow-leaved males and obtained an F1 consisting of males and females that were all broad leaved, but the F2 were of two kinds, broad-leaved and narrow-leaved.  Explain the type of sex determination system that exists in Lychnias alba.  SHOW YOUR WORK .
a- Type of system?
b- what
Genetics and Developmental Biology   rony   634   Asked 10 years ago
. You are studying a genetic disorder and find 63 affected siblings in a total
. You are studying a genetic disorder and find 63 affected siblings in a total
.  You are studying a genetic disorder and find 63 affected siblings in a total of 741. The population prevalence of the disorder has been estimated to be 0.0037.  Calculate the sibling recurrence risk. 
Genetics and Developmental Biology   CSDCUO   258   Asked 10 years ago
A certain human trait is determined by five unlinked pairs of alleles, with each
A certain human trait is determined by five unlinked pairs of alleles, with each
A certain human trait is determined by five unlinked pairs of alleles, with each dominant allele having an additive effect on the phenotype. 

A.  How many phenotypic classes would you predict, and in what frequencies, from a cross between two people who are heterozygous for all five loci? 


B.   How many genotypic classes would you predict?&
Genetics and Developmental Biology   CSDCUO   323   Asked 10 years ago
A cross was made to produce D. melanogaster flies heterozygous for two pairs of
A cross was made to produce D. melanogaster flies heterozygous for two pairs of
A cross was made to produce D. melanogaster flies
heterozygous for two pairs of alleles: A and a, which
determine long versus short wings, and B and b, which
determine gray versus ebony body color. The following F2
data were obtained:
Long wing, gray body 240
Long wing, ebony body 40
Short wing, gray body 40
Short wing, ebony body 80
Test
Genetics and Developmental Biology   bishoy93   420   Asked 9 years ago
A few doubts regarding Heart (Atrial) Embryology...
A few doubts regarding Heart (Atrial) Embryology...
HI GUYS  Waving Hand Sign

1. What is the developmental significance of both the Atrial appendages, i.e. what is the reason for their presence?  What I mean is, is it just to increase its volume as man has more oxygen and nutritional requirements all of a sudden in the developmental tree or what?

2. Why does septum spurium develop at all? It anyways is incorporat
Genetics and Developmental Biology   the13thhokage   1297   Asked 12 years ago
A mutation in which gene could be responsible for this biosynthetic auxotroph
A mutation in which gene could be responsible for this biosynthetic auxotroph
One of my mutants [EMS-mutagenized, ampicillin enriched, 99.9% killing] grew only [! only] on a plate containing arginine, aspartate, proline, glutamic acid, inositol, and ornithine. I've spent hours trying to find out where the mutation might be [i have found out how all the amino acids on the plate are related using ecocyc], but i can't pinpoint an intermediate that woul
Genetics and Developmental Biology   KLOB   742   Asked 11 years ago
A phenotypically normal woman is having a hard time getting pregnant. as her doctor, you run a karyo
A phenotypically normal woman is having a hard time getting pregnant. as her doctor, you run a karyo
a phenotypically normal woman is having a hard time getting pregnant. as her doctor, you run a karyotype and determine that sheis a carrier for a reciprocal translocation 4:20, while her husband's karyotype is normal

1. Explain her sub-fertility by drawing out all of her possible gametes

2. Now give chromosomal composition and phenotypes, with their expect
Genetics and Developmental Biology   lirun21319   1075   Asked 8 years ago
After a forest fire on the lower slopes of the Rockies, there are significant...
After a forest fire on the lower slopes of the Rockies, there are significant...
After a forest fire on the lower slopes of the Rockies, there are significant changes in the number and type of plant species found in the disturbed area. Describe how succession occurs in this plant community over the 150 years following a major fire.

Plants:  aspen, birch, and poplar trees. Dogwood, alder and willow are common scrubs. Riverside plants cattails, sed
Genetics and Developmental Biology   7dog47   1083   Asked 9 years ago
An AABbccDdEeFF individual is crossed with an individual with the genotype AaBBC
An AABbccDdEeFF individual is crossed with an individual with the genotype AaBBC
Question: An AABbccDdEeFF individual is crossed with an individual with the genotype AaBBCCDdEeff. What is the probability that their offspring will have the genotype AaBBCcddEEFf?

Can someone help and explain how to figure this out?
thanks,
Genetics and Developmental Biology   nsch22   478   Asked 10 years ago
An experiment was done to determine the linkage relationship of three genes (a,
An experiment was done to determine the linkage relationship of three genes (a,
An experiment was done to determine the linkage relationship of three genes (a, b, and c) in Drosophila melanogaster. Homozygous females phenotypically a, c were crossed with homozygous males phenotypically b. The F1 females were all wild-type in appearance and the F1 males were all a, c. The F1 females and males were crossed to give the following F2 phenotypes and numbers (SEE atta
Genetics and Developmental Biology   sagrawal1984   2082   Asked 9 years ago
Analyzing chromosome 1 of a male and female
Analyzing chromosome 1 of a male and female
1. The first child of this couple was Rh- (Rhesus negative). What is the chance that the second child of this is a girl and Rh- (Rhesus negative)? Use Punnett squares and/or the calculation to show your work.

2. Nomenclature for writing alleles on a homologous chromosome pair
 
The phrase DEP/dep means the first three letters, DEP, represent the order in
Genetics and Developmental Biology   helpmepassbio   479   Asked 6 years ago
Anyone have a download link for the solutions man.
Anyone have a download link for the solutions man.
Hi looking for a download link to the solutions book for Introduction to genetic analysis tenth edition
Genetics and Developmental Biology   Jthomas32090   3257   Asked 11 years ago
Are the blue blood vessels in the umbilical word labelled correctly?
Are the blue blood vessels in the umbilical word labelled correctly?
Please explain why it is labelled correctly or not,
Thank you so much.
Genetics and Developmental Biology   Thehobbitlass   1618   Asked 8 years ago
Assume that during meiosis I, none of the C chromosomes disjoin at metaphase, bu
Assume that during meiosis I, none of the C chromosomes disjoin at metaphase, bu
Assume that during meiosis I, none of the C chromosomes disjoin at metaphase, but they separate into dyads (instead of monads) during meiosis II. Each resultant gamete participated in fertilization with a normal haploid gamete. What combinations will result?


Check all that apply.
   A) two copies of chromosome A, two copies of chromosome B, four copies of chromos
Genetics and Developmental Biology   JaeYang   1294   Asked 9 years ago
At this time, million people in the U.S. population are suffering from mood-related disorders.
At this time, million people in the U.S. population are suffering from mood-related disorders.
At this time, ____________  million people in the U.S. population are suffering from mood-related disorders.

a. 1.5
b. 45
c. 60
d. 85
Genetics and Developmental Biology   cloveb   163   Asked 6 years ago
Calculate H2 for corolla length in Nicotiana.
Calculate H2 for corolla length in Nicotiana.
In Nicotiana, two inbred strains produce long (PL) and short (PS) corollas. These lines are crossed to produce F1, and the F1 are crossed to produce F2 plants in which corolla length and variance are measured. The table summarizes mean and variance of corolla length in each generation.


Generation  Mean Corolla Length   Variance
PL          
Genetics and Developmental Biology   tanker   3970   Asked 9 years ago
Calculate relatedness
Calculate relatedness
Is it possible to calculate the relatedness between family members(consanguineous) if you have the genotypes at all positions on te chromosomes.

Let me try to explain what I'm doing now:

A person can have 3 types of genotypes for example AA, BB and AB. If you combine 2 persons you will get a total of 9 different types of combinations.(AAAA,BBBB,ABAB,AAAB,B
Genetics and Developmental Biology   Thuur   685   Asked 10 years ago
Calculate the number of mismatches that could occur in one human cell during one
Calculate the number of mismatches that could occur in one human cell during one
Calculate the number of mismatches that could occur in one human cell during one round of replication. Assume the size of the human genome is 3.2 billion base pairs. Assume the error rate is 1 mismatch per 10^10 bp.
Express your answer using two significant figures.
Genetics and Developmental Biology   mki   1254   Asked 9 years ago
Calculate the number of mismatches that could occur in one human cell during one
Calculate the number of mismatches that could occur in one human cell during one
Calculate the number of mismatches that could occur in one human cell during one round of replication in the absence of mismatch repair. Assume that mismatch repair decreases the number of mutations by a factor of 1000.
Express your answer using two significant figures.
Genetics and Developmental Biology   mki   793   Asked 9 years ago
Calculating genotypes
Calculating genotypes
 A black body color (as opposed to the wild-type yellow color) in fruit flies is due to the recessive ebony allele, e. Small wings are due to the recessive vestigal allele, vg.  You wish to deduce the genotype of a yellow-bodied large-winged male fruit fly.  What are all the possible genotypes this male could be at the ebony and vestigal loci?  What is the genotype an
Genetics and Developmental Biology   Jessc   386   Asked 6 years ago
cDNA vs genomic libraries
cDNA vs genomic libraries
The first genomic library ever made was for the beta-globin gene taken from human genomic dna of the reticulocytes.
the library was done by using a partial digestion of the genome via restriction enzymes.
[ a partial digest is done by not allowing the reaction to go to completion achieved by limiting incubation time]
the fragments were then transferred to vectors of
Genetics and Developmental Biology   ehd123   654   Asked 10 years ago
cdna vs genomic library
cdna vs genomic library
The first genomic library ever made was for the beta-globin gene taken from human genomic dna of the reticulocytes.
the library was done by using a partial digestion of the genome via restriction enzymes.
[ a partial digest is done by not allowing the reaction to go to completion achieved by limiting incubation time]
the fragments were then transferred to vectors of
Genetics and Developmental Biology   ehd123   957   Asked 10 years ago
Cell-based manufacturing in animal cell tissue culture
Cell-based manufacturing in animal cell tissue culture
I am interested to gain more knowledge  on this topic. I be really grateful if you guys can provide any information regarding this topic (books, journals...etc). Your feedback is highly appreciated. Slight Smile
Genetics and Developmental Biology   Yutadono   276   Asked 7 years ago
chromosomal basis question Please Help :)
chromosomal basis question Please Help :)

For an X-linked recessive trait, human females must be ___ for the recessive allele in order to express the trait.

1.hemizygous
2.homozygous
3.heterozygous
4.heterogametic
 
Humans with Turner syndrome are

1.polyploid.
2.aneuploid.
3.heteroploid.
4. Haploid.
Genetics and Developmental Biology   fanaan   1053   Asked 10 years ago
Chromosome Problems
Chromosome Problems
Consider a diploid cell where 2n = 6. During metaphase I of meiosis, as the pairs of homologous chromosomes line up on the metaphase plate, each pair may orient with its maternal or paternal homolog closer to a given pole. There are four equally probable arrangements of the homologous pairs at metaphase I. (Note that this problem assumes that no crossing over has occurred.)
Genetics and Developmental Biology   BriannaG1996   531   Asked 9 years ago
Complementation problem: possible region of deletion place the mutation on the thick line which ...
Complementation problem: possible region of deletion place the mutation on the thick line which ...
I need help with part B of this problem, I've asked Genetics tutors, other professors and even went on chegg to use the expert Q&A --  I've done part A and I know that 3 complements 1, 2 and 6 are unable to complement and 3,4,5 and 1,2 6 have similar mutations

but I have no idea if I am right?
my genetics professor says that if I look at row 4 and
Genetics and Developmental Biology   exlax2   260   Asked 6 years ago
Creation of CFTR Knockout Embryonic Stem Cells by Positive-Negative Selection
Creation of CFTR Knockout Embryonic Stem Cells by Positive-Negative Selection
I am having trouble understanding what is going on here. What I got thus far is that the more preferred fate is Homologous recombination (targeted approach). Next, you basically plate the ES cells that are both neomycin & ganciclovir resistant (homologous recombination). I basically do not know if I am on the right track. Any feedback of any sort will help.
Genetics and Developmental Biology   willuhelp   369   Asked 10 years ago
Creation of CFTR Knockout Mice by Injection of KO ES Cells into a Blastocyst
Creation of CFTR Knockout Mice by Injection of KO ES Cells into a Blastocyst
I'm really having a tough time with this KO mouse concept. If anyone could explain what is going on in each step, this would really help me.
Genetics and Developmental Biology   willuhelp   423   Asked 10 years ago
Crossing lines with the same recessive phenotype What do you get from the result
Crossing lines with the same recessive phenotype What do you get from the result
Gene Interactions
You study color variants of Arabidopsis hypotheticus, a plant with red flowers. You have obtained three pure-breeding mutant lines, all named for their place of origin. Two lines have white flowers (Aberdeen White and Victoria White), and one has yellow flowers (Kansas Yellow).
You begin your analysis by crossing each line with wild-type plants and selfin
Genetics and Developmental Biology   Nour   4683   Asked 9 years ago
Deletions, insertion, and frameshift mutations are more serious than a point
Deletions, insertion, and frameshift mutations are more serious than a point
I got the following questions wrong on a test/assignment.  Can someone let me know what the correct answers are so I have the correct information for my final exam. Thx

#1   Deletions, insertions, and frameshift mutations are more serious than a point mutation because
A.  mis-sense mutations result in more malfunction than nonsense mutations
B.&nb
Genetics and Developmental Biology   slim16   748   Asked 7 years ago
Design a yeast strain that would turn blue when fed...... Please help!
Design a yeast strain that would turn blue when fed...... Please help!
1.   Design a yeast strain that would turn blue when fed medium that contains hydrolyzed rice and beans (hydrolysis breaks proteins down to their constituent amino acids) plus threoninol and X-gal.  Draw the structure of the gene/mRNA that will give the yeast this phenotype and describe the molecular events that lead to the yeast turning blue on medium containing hydrolyzed rice
Genetics and Developmental Biology   fecster   553   Asked 9 years ago
Did I answer these correctly?
Did I answer these correctly?
1. A diploid somatic cell from a pigeon (Columba livia) has a total of 80 chromosomes (2n = 80).
Chickens have the ZZ-ZW chromosomal sex determination. What is the total number of chromosomes present in a single cell during prophase II of meiosis?
a. 80 ANSWER
b. 40
c. 160
d. 120
e. 20

2. In the pigeon example above, what is the total number
Genetics and Developmental Biology   MikeMello   1047   Asked 9 years ago
Distance between loci
Distance between loci
I was wondering whether the distance between loci ever change?

 For example if eye color was on gene A and pupil size on gene B, the distance between gene A and gene B is 50 cM. Is there a scenario where A and B is not 50?

My teacher said it can, but I don't understand.  Isn't the distance between certain genes always fixed?
Genetics and Developmental Biology   sokho   375   Asked 10 years ago
Distance between shine delgarno and start codon affecting mRNA requirement?
Distance between shine delgarno and start codon affecting mRNA requirement?
Here's a question that I can't seem to grasp.

There are two mRNAs presented in the 5' to 3' direction, one of which belongs to strain A and the other one belonging to strain B.

Strain A's shine delgarno is further away from the AUG start codon (~17nucleotides)
Strain B's shine delgarno is optimal distance from the AUG start co
Genetics and Developmental Biology   Chadori   393   Asked 9 years ago
DNA Statements Question
DNA Statements Question
Which of the following statements is NOT true?
 
a)DNA is composed of deoxynucleotides whereas RNA is composed of ribonucleotides.
b)Deoxyribose is a ribose with a missing oxygen atom at the 3’-carbon atom.
c)The A-T pairing is stronger than the G-C pairing.
d)The DNA backbone consists of covalent bonds between sugars and phosphate groups.
e)The typica
Genetics and Developmental Biology   diamond3094   726   Asked 10 years ago
DNA Synthesis: heated and cooled, fregments & total DNA. hybridized? Help!
DNA Synthesis: heated and cooled, fregments & total DNA. hybridized? Help!
1-   During DNA synthesis, small fragments of DNA are formed.  They were collected and combined with total DNA.  The mixture was heated and cooled down.  What would you expect to happen?
a.   The small fragments would hybridize together
b.   The small fragments would not hybridize to native DNA
c.   The small fragments would hybridize to the leading strand; the
Genetics and Developmental Biology   TinkerBell2175   407   Asked 9 years ago
Draw a pedigree. Probability of 1st child to have sickle cell anemia?
Draw a pedigree. Probability of 1st child to have sickle cell anemia?
The autosomal gene for sickle cell anemia (ss) is prevalent among East Africans. Affected individual shave elongated and curved erythrocytes under low oxygen tension because of the polymerization of hemoglobin. Supposing heterozygotes for this gene in this population occur in a frequency of 1 in 8. If a phenotypically normal guy marries a second wife who is also phenotypically norma
Genetics and Developmental Biology   phyllidiela   386   Asked 9 years ago
Dual Luciferase Reporter Gene Assays
Dual Luciferase Reporter Gene Assays
The accuracy of reporter assays can be improved by utilizing a dual reporter system. One of the reporter genes is correlated with the promoter of interest and is used to assess the effects of specific experimental conditions, while the second reporter is used as a control and serves as a baseline response. The Super Light™ Dual- Luciferase Reporter Assay allows for the sequential me
Genetics and Developmental Biology   MarkHolland   493   Asked 11 years ago
EMERGENCY: Need help with UMUC Lab # 5 Genetic Inheritance
EMERGENCY: Need help with UMUC Lab # 5 Genetic Inheritance
Lab # 5
Genetic Inheritance

Section A: Patterns of Inheritance

Background Information
In the 19th century, a monk named Gregor Mendel conducted a series of experiments to determine how ‘traits’ or features of pea plants were passed on from generation to generation.  He carefully studied seven traits of successive generations of pea plants to disc
Genetics and Developmental Biology   Dave11   1372   Asked 10 years ago
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