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Can pGEM-T Easy Vector JM109 cells take up multiple copies of the Plasmid?
I am using JM109 cells and the pGEM-T easy vector system II to clone a mixed microbial community for sequencing and I need to know whether a sinle copy or multiple copies can be taken up by each competent cell because this will impact on my ability to sequence from purified plasmid from 1 white cell.
What would be the size in base pairs of the largest and smallest amplicons that would be expected if
Relevant details of the STRs are as follows
TH01
Chromosomal location: 11p15.5 (tyrosine hydroxylase, 1st intron) Repeat motif: TCAT (GenBank top strand) although AATG (bottom strand) often used. Note that variants with a [AATG]nATG[AATG]n structure quite common e.g. [AATG]6ATG[AATG]3 would be 9.3 repeats. Allele range: 3-14
Successful PCR experimentation depends crucially on primer design and characterisation. Poor primer design can lead to a series of problems that can result in, for example, no amplification, poor amplification or the amplification of multiple targets.
What are the type of questions on the MCAT? Are all conceptual based on readings or calculations too?
I was wondering if it is comparable to a Chemistry class, for example (tedious calculations, formulas, etc.). Do questions require formulas to use and are these formulas provided or expected to be known? I was wondering if it is comparable to a Chemistry class, for example (tedious calculations, formulas, etc.). Do questions require formulas to use and are these formulas provided o
Biomechanics - Exercise and Sports Medicine. What is the half-life
This is a following sample question from a past midterm. The midterm is later this morning (11 am eastern time) so help would appreciated!
Question. A tendon with a maximum strain of 10% and a passive visco-elastic half-life of 5 minutes for both elongation and recovery from elongation is stretched by a load sufficient to cause it to rupture if left to creep indefinitely.
Capital budgeting is intended to do which of the following?
Capital budgeting is intended to do which of the following?
A) assist financial managers with balancing income and expenses B) help firms determine the most profitable global expansion project C) allow firms to minimize overhead expenses for global projects D) eliminate the need for international accounting experts
You’re walking around the deserts of Arizona looking for a pet snake to add to your ...
You’re walking around the deserts of Arizona looking for a pet snake to add to your collection. You encounter a very pretty specimen that shows a yellow, black, and red striped pattern. It’s very friendly for a reptile, but you notice the red stripes touch the yellow stripes
a). Is it safe to collect? b). Why or why not? c). What’s the common name of this speci
For each statement, the distribution of a population is illustrated before a selective force causes ...
For each statement, the distribution of a population is illustrated before a selective force causes the population to evolve.
i. Indicate where on the graph the selection takes place (it could be one place or two) by placing an “x” (or two if necessary). ii. Draw the new population distribution on the graph. iii. Indicate the type of selection taking place.
One way to ensure that a person is inputting the correct information in the correct format is to use a(n) ________ list, such as a list of vehicle models.
A ball is thrown straight up from a 20.0 meter cliff at 17.5 m/s. the ball goes up and then all the ...
A ball is thrown straight up from a 20.0 meter cliff at 17.5 m/s. the ball goes up and then all the way to the ground below the cliff. Find: How high did the ball go and how fast was it going when it hit the ground? Up Down xi= &n
An Air Force jet lands on a straight runway with a forward velocity of 75 m/s and the pilot applies ...
An Air Force jet lands on a straight runway with a forward velocity of 75 m/s and the pilot applies the brakes at a constitutional rate for 20 seconds until the jet slows to 10 m/s. How far down the runway did the jet go during this time?
Searching for program capable systematize protein functions
Hello. I have list of 50 proteins (with accession numbers). My goal is to use some program to where I can type list of accession numbers and to expect from program to give me some charts (e.g. pie charts) showing such things as intracellular localization (what compartment), functions (e.g. peroxidase activity, proinfllamatory response) e.t.c. Please let me know if you know suc
You are at a party, and you hear a crash. Two people are helping up a friend who
You are at a party, and you hear a crash. Two people are helping up a friend who fell and knocked over q small end table. He does not appear hurt, but you notice he is staggering and mumbling incoherently. " He 's not bad when he's drunk, but he should stay off the ecstasy. He gets really stupid. Les's see if we can put him to bed to sleep it off" 1. sh
You are at a large public gathering and a person suddenly cries out, falls...
What would you do? 1.You are at a large public gathering and a person suddenly cries out, falls, becomes rigid, and has muscular jerks. He has a frothy saliva on the lips and very shallow breathing. In about five minutes, the symptoms subside, and within the next thirty minutes the symptoms reappear and subside numerous times. 2.At the same gathering, a person complains of
You are at a local park when a family at the pavilion begins to yell and...
You are at a local park when a family at the pavilion begins to yell and scream for help. You run over and see a man in his mid-forties lying in a puddle of water next to the electric cooler. A family member indicates that the man went to plug in the cooler when a loud pop was heard and a large spark was seen. The man is not moving. You note a burn to the hand with the cord in it an
As you are driving to work, you come across a car accident. You find one...
As you are driving to work, you come across a car accident. You find one person outside of his crashed vehicle, on the ground, leaning against the back bumper. He is bleeding firm the head and says his knee hurts. You notice a strong smell of gasoline. Looking under the vehicle, you see a growing pool of what you believe to be gasoline. 1.Is the victim a candidate for an emerg
You are out for an early spring morning jog in the local park when you come...
You are out for an early spring morning jog in the local park when you come across a man in his mid -forties lying on a park bench who appears to be unresponsive. As you approach him, you notice his skin is extremely pale with cyanosis to his face and neck, and his breathing is extremely shallow and slow. He is dressed lightly in a tee shirt and cotton pants with shoes and no socks.
Molecules that are eaten but not absorbed by the animal leave the animals body i
Molecules that are eaten but not absorbed by the animal leave the animals body i Answer it Table I:Mealtime Grains Fruit Vege... Answer it hi, does any body know the yogurt produc... Answer it Explain how the structure of a carbohydr... Answer it How has recent evidence that birds are d... Answer it -------------
After a forest fire on the lower slopes of the Rockies, there are significant...
After a forest fire on the lower slopes of the Rockies, there are significant changes in the number and type of plant species found in the disturbed area. Describe how succession occurs in this plant community over the 150 years following a major fire.
Plants: aspen, birch, and poplar trees. Dogwood, alder and willow are common scrubs. Riverside plants cattails, sed
Briefly explain the following giving relevant questions, if necessary. The answers for this questio
briefly explain the following giving relevant questions, if necessary. The answers for this question should not be more than 1 1/2 pages in length.
sensitivity and selectivity Explain the following: i) calibration sensitivity ii) analytical sensitivity iii) selectivity iv) the difference(s) between selectivity and sensitivity
Rank the solvent systems best to worst in SN1 reactions.
The solvent systems are: 1:1 ethanol: water, 1:1 methanol: water, 1:1 1-propanol: water, and 1:1 acetone:water. Which solvents work from best to worst in SN1 reactions and why?
How to calculate experiment yield and theoretical yield for this problem?
If a student were to recover 22% trimyristin from a 10.0g sample of nutmeg, answer the following questions: 1. What is the experimental/actual yield of trimyristin from this solid/liquid extraction? 2. If you utilized a saponification (hydrolysis) reaction to generate myristic acid and glycerol from the recovered trimyristin (see hydrolysis reaction scheme below), what w
When a therapist enthusiastically subscribes to one treatment orientation but then uses therapeutic ...
When a therapist enthusiastically subscribes to one treatment orientation but then uses therapeutic techniques associated with other theoretical orientations, he or she is said to be:
a) Unreliable b) Eclectic c) Generic d) Ambivalent
Question 2
Binge eating disorder is more responsive to treatment than a
Please assist with the following questions (Engine Diagnostics)
10. A vehicle fails an HC emission test at idle and 2,500 rpm, and the engine has an acceleration stumble. The heated oxygen sensor (HO2S) voltage is 0.1–0.3 volts on a scan tool, and the injector pulse width is high. No diagnostic trouble codes (DTCs) are found. When propane is directed into the air intake, HO2S voltage immediately increases and the injector pulse width decr
Does decreased cardiac amount mean accumulation of blood to tissues/cells?
I'm trying to understand cardiac output. So, to my knowledge, decreased cardiac output = less blood being ejected by the heart/ventricles per minute, right?
What does that mean, specifically? Does that mean more blood is accumulated somewhere else? Like in the tissues/cells..lungs?
If blood is accumulated somewhere else...for example the kidneys ..doe
A device that monitors the activity of the photoreceptor cells of the eye indicates that there is a constant flow of neurotransmitter being released by the photoreceptor cells. This information implies that the subject is A. reading. B. in a dark room. C. outside in sunlight. D. in a brightly lit auditorium. E. focusing on a distant object.
Trying to check some questions, any help is appreciated
1)Meninges are ______. A. Bacterial infections of the CNS B. Connective tissue coverings around the central nervous system C. Synapses between CNS neurons and the post-synaptic membranes of other neurons D. Non-neuronal cells in the CNS that help regulate ionic concentrations of their ECF (my guess is B)
25ml of a 0.388M solution of sodium sulphate is mixed with 35.3 ml of 0.229M sodium sulphate .what is the molarity of the resulting solution? Assume the volume are additive
