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Distance between shine delgarno and start codon affecting mRNA requirement?
Here's a question that I can't seem to grasp.
There are two mRNAs presented in the 5' to 3' direction, one of which belongs to strain A and the other one belonging to strain B.
Strain A's shine delgarno is further away from the AUG start codon (~17nucleotides) Strain B's shine delgarno is optimal distance from the AUG start co
The GTP binding domain of elongation factor Tu (EF-Tu) and elongation factor 1α
The GTP binding domain of elongation factor Tu (EF-Tu) and elongation factor 1α (EF-1 α) contains an eight amino acids conserved region, with the sequence FIKNMITG (using the one letter code). Here you are designing only one of the two primers required for PCR.
a. If you were using this region to design a primer to amplify by PCR the sense strand of EF-Tu/EF-1
DNA Synthesis: heated and cooled, fregments & total DNA. hybridized? Help!
1- During DNA synthesis, small fragments of DNA are formed. They were collected and combined with total DNA. The mixture was heated and cooled down. What would you expect to happen? a. The small fragments would hybridize together b. The small fragments would not hybridize to native DNA c. The small fragments would hybridize to the leading strand; the
How to solve lac operon problem? HW due tomorrow midnight!! ><
Hello!
I was wondering if someone can explain how to solve a lac operon problem? The attached picture is the one from our problem sets and the answers are right, I just don't understand how to get the answers.
A cross was made to produce D. melanogaster flies heterozygous for two pairs of
A cross was made to produce D. melanogaster flies heterozygous for two pairs of alleles: A and a, which determine long versus short wings, and B and b, which determine gray versus ebony body color. The following F2 data were obtained: Long wing, gray body 240 Long wing, ebony body 40 Short wing, gray body 40 Short wing, ebony body 80 Test
Please help me answer this question. AaBbCc x aabbcc test cross with ABC linked and dominant. 2000 phenotypes observed with the following individuals counted out of 2000. establish the gene arrangement and map distances.
Given the pedigree below, what is the probability...?
The pedigree illustrated below (see attached jpeg file) shows a rare autosomal recessive trait. What is the probability that a child of III-4 and III-5 will manifest the phenotype for this trait?
In cats, an x-linked pair of alleles, B and B', determines the color of fur. The allele "B" for yellow is codominant with B' for black so that BB' cats are tortoise shelled, a splotchy mixture of yellow and black hairs. You and a friend are strolling down the street and see a tortoise shell cat. You bet your
Where does the cut take place in palindrome sequence
In the case of restriction enzymes, I know that it chooses Palindromes, but where does the exact cut take place? Is it always between A and T base pairs or Can it occur between any base pairs in a palindrome sequence.
someone put only the answers but would you put it the full answer please instead only putting A,B Thanks. Case Studies: Pediatrics: Congenital Heart Disease: Billy Adams
Indicate whether each of these is plesiomorphic (ancestral) or apomorphic (derived) in the great ape lineage, using the phylogeny below. Great apes include orangutan, gorilla, chimp, and human.
The likelihood of having identical children (who are not twins)
So I figure if you have enough babies (probably in the billions) you'd eventually get one that was essentially genetically and/or aesthetically identical to another. Could anyone give me any background information and numbers regarding the likelihood of this?
How many possible alleles are at a locus consisting of 3 base-pairs in a diploid
ugh, I am so confused...
So, I guess a locus is just a spot for a gene, so the gene is also considered to consist of 3 bases? Gene: _ _ _ Each "_" could be either an A,T,C, or G. So there are 4 possibilities per "_" So, there are 4 x 4 x 4 = 64 different possible alleles of a gene on one chromosome...? Since there are 2 c
Can someone please tell me what is the formulae for calculating genetic map units?
Here is the question from my quiz:
The cross GE/ge × ge/ge produces the following progeny: GE/ge 404, ge/ge 396, gE/ge 97, Ge/ge 103. From these data, one can conclude that there are 20 map units between the G and E loci.
1. The ability to curl one’s tongue into a U-shape (Q) is dominant to noncurlers (q). A curler woman has a father who is a noncurler. She has five children with a noncurler man. What is the probability that four children are curlers and the other is a noncurler?
2. Your lab is investigating the genetics of a new species of beetle. You have isolated a truebreeding line tha
In green turtles, an odd number of plates on there shell is dominant over an even number of plates. On the same chromosome is a gene for ear spot color with red being dominant over orange. On this same chromosome is the gene for strip color on the body with yellow being incompletely dominant with blue strips. These turtles were crossed in a breeding experiments and the following dat
Use the following table of progeny phenotypes for 7 different deletions
Deletions can be used to map genes along a chromosome. In order to do this a series of crosses in which one parent is homozygous for a mutant allele is crossed with the other parent that is homozygous for a partial deletion of the region. The progeny are scored to determine whether they have the mutant phenotype ("m") or the wild-type phenotype ("+"). If a mutati
I can't figure out how to do the punnet square for any of this or what it all means? Can anyone please explain how to understand the solution? Looks like a bunch of letters and numbers to me.
Red-green color-blindness is an X-linked recessive trait in humans. Polydactyly (extra fingers and toes) is an autosomal dominant trait. Martha has normal fingers and toe
An AABbccDdEeFF individual is crossed with an individual with the genotype AaBBC
Question: An AABbccDdEeFF individual is crossed with an individual with the genotype AaBBCCDdEeff. What is the probability that their offspring will have the genotype AaBBCcddEEFf?
Can someone help and explain how to figure this out? thanks,
1. The first child of this couple was Rh- (Rhesus negative). What is the chance that the second child of this is a girl and Rh- (Rhesus negative)? Use Punnett squares and/or the calculation to show your work.
2. Nomenclature for writing alleles on a homologous chromosome pair
The phrase DEP/dep means the first three letters, DEP, represent the order in
. In rye grass, seed color is a polygenic trait (additive model). If true-breeding red and white varieties are crossed, the F1 are intermediate in color. If the F1 are self-fertilized, about 1 in 64 have white seeds. If the F1 are testcrossed, how many different genotypic categories will be produced?
The accuracy of reporter assays can be improved by utilizing a dual reporter system. One of the reporter genes is correlated with the promoter of interest and is used to assess the effects of specific experimental conditions, while the second reporter is used as a control and serves as a baseline response. The Super Light™ Dual- Luciferase Reporter Assay allows for the sequential me
Help with Identificatying Restriction Enzyme Recognition Sites in a DNA sequence
Hi Folks,
I'm really struggling with a PCR & restriction enzyme question that's part of my formative (unmarked) work for the summer months in preparation from an Applied Biology course at University.
The entire set of questions are set out on an instruction sheet so I'll try to replicate them here as I'm struggling to fully understand ho
Consider a diploid cell where 2n = 6. During metaphase I of meiosis, as the pairs of homologous chromosomes line up on the metaphase plate, each pair may orient with its maternal or paternal homolog closer to a given pole. There are four equally probable arrangements of the homologous pairs at metaphase I. (Note that this problem assumes that no crossing over has occurred.)
Identify the most likely mode of inheritance found in this pedigree
I think that the most likely mode would be autosomal dominance with incomplete penetrance because it did skip one generation. Any help would be greatly appreciated. Let me know what you think it is and please also write why you think it is that. Thanks!
What would be the size in base pairs of the largest and smallest amplicons that would be expected if
Relevant details of the STRs are as follows
TH01
Chromosomal location: 11p15.5 (tyrosine hydroxylase, 1st intron) Repeat motif: TCAT (GenBank top strand) although AATG (bottom strand) often used. Note that variants with a [AATG]nATG[AATG]n structure quite common e.g. [AATG]6ATG[AATG]3 would be 9.3 repeats. Allele range: 3-14
The question asks to calculate the tandem repeats in the D1S80 allele. i plotted the graph but im not sure what to do from there. next question asks to be figure outhow many diffferent classes of homozygous and heterozygous exist? any help will be greatly appreciated
Design a yeast strain that would turn blue when fed...... Please help!
1. Design a yeast strain that would turn blue when fed medium that contains hydrolyzed rice and beans (hydrolysis breaks proteins down to their constituent amino acids) plus threoninol and X-gal. Draw the structure of the gene/mRNA that will give the yeast this phenotype and describe the molecular events that lead to the yeast turning blue on medium containing hydrolyzed rice
