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-20 microbial glycerol stocks
-20 microbial glycerol stocks
hello,

I am keeping microbial stocks in 25% glycerol at -20 (-80 not available) for a few months. but I am noticing that the stocks are remaining liquid. will this affect the viability of the cells ? if so what can I do without repeating the whole process again. thank you
Upper-Year Courses   tahah   1328   Asked 7 years ago
. You are studying a genetic disorder and find 63 affected siblings in a total
. You are studying a genetic disorder and find 63 affected siblings in a total
.  You are studying a genetic disorder and find 63 affected siblings in a total of 741. The population prevalence of the disorder has been estimated to be 0.0037.  Calculate the sibling recurrence risk. 
Genetics and Developmental Biology   CSDCUO   258   Asked 10 years ago
1.(1 point)Suppose that in the British compound of a POW camp, the price of chee
1.(1 point)Suppose that in the British compound of a POW camp, the price of chee
1.(1 point)Suppose that in the British compound of a POW camp, the price of cheese is 6 cigarettes per cheese ration and price of coffee beans is 3 cigarettes per coffee ration. In the French compound, the price of coffee beans is 24 cigarettes per ration.
1)Is there an opportunity for beneficial exchange? no
2)A British prisoner could exchange his cheese ration for ( &nbs
Other   JJULIET42   4166   Asked 9 years ago
1.50 moles of an ideal monatomic gas are initially at a temperature of 317 K. If
1.50 moles of an ideal monatomic gas are initially at a temperature of 317 K. If
1.50 moles of an ideal monatomic gas are initially at a temperature of 317 K. If the gas gains 2730 J of heat and performs 780 J of work, what is its final temperature?
A) 359 K
B) 421 K
C) 526 K
D) 687 K
E) 756 K
Chemistry   PPK   2109   Asked 12 years ago
1.Throughout the years, theorists and educators have expressed different perspe
1.Throughout the years, theorists and educators have expressed different perspe
Throughout the years, theorists and educators have expressed different perspectives about children. Discuss how the changing views have influenced the learning environment, then and now.
Other   Jennyadams   856   Asked 8 years ago
100-kg thin circular hoop has radius 2 m. Its angular velocity decreases from 20 rad/s to zero in
100-kg thin circular hoop has radius 2 m. Its angular velocity decreases from 20 rad/s to zero in
100-kg thin circular hoop has radius 2 m. Its angular velocity decreases from 20 rad/s to zero in 200 s due to friction. What is the frictional torque acting on the hoop?

(1) 40 N-m (2) 20 N-m (3) 16 N-m (4) 27 N-m (5) None of these.

PHY2053, Summer 2012 Exam
Physics   cloveb   132   Asked 6 years ago
150 grams of cod measured 0.11 ppm of mercury,how many mg in 150 grams
150 grams of cod measured 0.11 ppm of mercury,how many mg in 150 grams
150 grams of cod measured 0.11 ppm of mercury,how many mg in 150 grams
Chemistry   gllendadobson   998   Asked 9 years ago
17.A 45.5-lb carton is initially at rest. It is then pulled horizontally by a 19.4-lb force in the
17.A 45.5-lb carton is initially at rest. It is then pulled horizontally by a 19.4-lb force in the
17.   A 45.5-lb carton is initially at rest.  It is then pulled horizontally by a 19.4-lb force in the direction of motion and is resisted by a frictional force which is equal (in pounds) to four times the carton’s velocity (in ft/s).  Find the differential equation of motion.


Mathematics   mathinator   216   Asked 6 years ago
19. To a Canadian, the statement “I am who I am, regardless of who my family is”
19. To a Canadian, the statement “I am who I am, regardless of who my family is”
yep
Psychology   Smartseve   209   Asked 7 years ago
1d. determine the number of grams of water required at 100 Celsius to dissolve a
1d. determine the number of grams of water required at 100 Celsius to dissolve a
1d. determine the number of grams of water required at 100 Celsius to dissolve a mixture containing 20 g KNO3 and 2.0 g CuSO4*5H20 assuming that the solubility of one substance is not affected by the presence of another.

2. to the solution in Problem 1d at 100 Celsius, 15g of water are added and the solution is cooled to 0 Celsius

Chemistry   sesait   3856   Asked 12 years ago
1st Ig isotype produced during cource of 1° imm.response has which heavy chain?
1st Ig isotype produced during cource of 1° imm.response has which heavy chain?
A.   γ
B.   κ
C.   δ
D.   μ
E.   None of the above

Cell Biology   evan888   328   Asked 10 years ago
1st International Student Congress of Evolutionary Biology
1st International Student Congress of Evolutionary Biology
The first student congress about evolutionary biology is being organized by Middle East Technical University - Biology and Genetics Students Club.

Dates: 29 September - 1 October 2012
Place: Middle East Technical University, Culture & Congress Hall, Ankara/TURKEY

Please visit our web page!

isceb.org
Off-Topic Room   Guard   850   Asked 11 years ago
2 ap biology multiple choice help needed?
2 ap biology multiple choice help needed?
A component of the body?s second line of defense does not include

 
   cell-mediated apoptosis  
 an inflammatory response to recent injury
 phagocytes ingesting invading organisms
 anti-microbial activity due to lysozyme excreted in tears  
 
Viral infected cells sometimes secrete a substance called interferon, which diffuses to other cells and protects them against viral infec
High School Level Science   rk90   839   Asked 11 years ago
2-D gel electrophoresis Animation
2-D gel electrophoresis Animation
Check out this animation on 2-D Gel Electrophoresis!

<a rel="nofollow" href="https://http://glencoe.mcgraw-hill.com/sites/dl/free/9834092339/291136/f21_04_2d_gel_electroph.swf" target="_blank">https://http://glencoe.mcgraw-hill.com/sites/dl/free/9834092339/291136/f21_04_2d_gel_electroph.swf</a>
Other   sarah   11555   Asked 13 years ago
2.50 moles of a monatomic ideal gas expand adiabatically from an initial tempera
2.50 moles of a monatomic ideal gas expand adiabatically from an initial tempera
2.50 moles of a monatomic ideal gas expand adiabatically from an initial temperature of 300 K and an initial volume of 80.0 L to a final volume of 150 L. What is the final temperature of the gas?
A) 840 K
B) 563 K
C) 426 K
D) 287 K
E) 197 K
Chemistry   PPK   1445   Asked 12 years ago
2.What are social characteristics of today’s prisoners? What gender and racial
2.What are social characteristics of today’s prisoners? What gender and racial
What are social characteristics of today’s prisoners? What gender and racial disparities, if any exist in today’s prison populations?
Legal Studies   shelle001   667   Asked 9 years ago
20 cancer cases in French women with PIP implants: agency
20 cancer cases in French women with PIP implants: agency
French health authorities said Friday that 20 cases of cancer had been uncovered in women with allegedly faulty French-made breast implants but insisted there was still no proven link with the disease.

French consumer health agency AFSSAPS said that as of December 28 it had registered 15 cases of breast adenocarcinoma, the most frequent form of breast cancer, one c
News Articles and Discussion   bio_man   960   Asked 12 years ago
20.0 L of a monatomic ideal gas at a pressure of 100 kPa expand adiabatically un
20.0 L of a monatomic ideal gas at a pressure of 100 kPa expand adiabatically un
20.0 L of a monatomic ideal gas at a pressure of 100 kPa expand adiabatically until the volume doubles. What is the pressure in the gas at that point?
A) 31.5 kPa
B) 50.0 kPa
C) 200 kPa
D) 317 kPa
E) 400 kPa
Chemistry   PPK   727   Asked 12 years ago
3.0 moles of gas expand from an initial volume of 0.040 m3 to a final volume of
3.0 moles of gas expand from an initial volume of 0.040 m3 to a final volume of
3.0 moles of gas expand from an initial volume of 0.040 m3 to a final volume of 0.085 m3 while the temperature of the gas is kept fixed at 300 K. How much work is done by the system?
A) 5.6 kJ
B) 6.6 kJ
C) 7.6 kJ
D) 8.6 kJ
E) 14 kJ
Chemistry   PPK   1625   Asked 12 years ago
4 parameter logistic fit for PCR data
4 parameter logistic fit for PCR data
I have a question about the logistic fit they used on this paper: onlineDOTliebertpubDOTcom/doi/pdf/10.1089/cmb.2005.12.1047. Its PDF page 5, or journal page 1051. They define all the variables and then say x0 and b define the shape of the model. If I am fitting 30 cycles of PCR data how am I suppose to know what to use for x0 and b?
Mathematics   requiem31   1149   Asked 9 years ago
45 of 60
45 of 60
If you were a travel agent with clients who wanted an ecotour to see muskox, polar bears, wolves, and lemmings, where would you send your clients.
A.  Article tundra
B.  taiga
C.  Grassland
D. Alpine tundra
E.  desert

If you wished to compare the efficiency of energy flow in two biomes with plants and animals of similar lifest
General Biology   Babylacy   812   Asked 12 years ago
5.The textbook discusses three levels of variances, Level 0, Level 1, Level 2, and Level 3. Briefly
5.The textbook discusses three levels of variances, Level 0, Level 1, Level 2, and Level 3. Briefly
What are each level of variances in cost accounting?
Accounting   JAlexandre   440   Asked 7 years ago
5.Using the terms provided, complete the statements below. Some terms may apply
5.Using the terms provided, complete the statements below. Some terms may apply
5.   Using the terms provided, complete the statements below. Some terms may apply more than once, while others may not apply at all. (15 points)

neutrophils   B cells   cytokines   heavy chains
histone   Toll-like receptors   chromatin   AIDS
light chains   gene rearrangements   dendritic cells   phagocytosis
macrophages   mast cells   antibodies   T cells
helper T cells   HIV   h
General Biology   jje5027   430   Asked 9 years ago
50 Integumentary Health Problems with Answers and Rationales
50 Integumentary Health Problems with Answers and Rationales
This is a test drill for Integumentary Health Problems
The questions are in line with the framework of Nurse Licensure Exam and derived from Saunders NCLEX Review 3rd
edition.They are common board questions for you to study
Next post will be the answers and rationales. You're all free to post a comment and rep me up.
Good luck guys and have fun with the test
Nursing and Clinical   biobio   20267   Asked 11 years ago
50 Medical Surgical Nursing Problems with Answers and Rationales
50 Medical Surgical Nursing Problems with Answers and Rationales
This is a test drill on Medical Surgical Nursing
The questions are in line with the framework of Nurse Licensure Exam and derived from Lippincot's and Springhouse Review Book.
These are common board questions for you to study
Next post will be the answers and rationales. You're all free to post a comment and please rep me up.
Good luck guys and have fun
Nursing and Clinical   biobio   6582   Asked 11 years ago
50 year war on Poverty
50 year war on Poverty
Hi Everyone, I am looking for some kid friendly reading and statistics for my 8th grade algebra students to read on the "50 year war on Poverty." Does anyone have a good website that you could recommend that is bipartisan that students can view to get some information about the statistics? (we are working on percent of change, rate of change, interpreting slope, etc.) I re
Teaching and Learning Strategies   benji   572   Asked 10 years ago
700 J of heat are added to 12 moles of an ideal monatomic gas at constant volume
700 J of heat are added to 12 moles of an ideal monatomic gas at constant volume
700 J of heat are added to 12 moles of an ideal monatomic gas at constant volume. What is the change in temperature?
A) 4.7 K
B) 5.2 K
C) 5.8 J
D) 6.8 K
E) 9.3 K
Chemistry   PPK   1036   Asked 12 years ago
75° W- what time would a person in Boston see the Sun on the meridian?
75° W- what time would a person in Boston see the Sun on the meridian?
If a person at 75 degrees W sees the Sun on the meridian at noon, at what time would a person in Boston see the Sun on the meridian?
Physics   sciencehelp95   2378   Asked 9 years ago
8 step reseach model described by Neil J Salkind
8 step reseach model described by Neil J Salkind
I need some articles or pdfs on this subject...email them to me @ sistermathisspeaks@yahoo.com
Teaching and Learning Strategies   Sister Mathis   544   Asked 8 years ago
8-Year-Olds Publish Article in Respected Biology Journal
8-Year-Olds Publish Article in Respected Biology Journal
A class project about bees prepared by a group of British schoolchildren was published in a leading scientific journal Wednesday.

The 25 students -- aged between eight and 10 -- studied how bees see color and patterns for a class project at Blackawton Primary School in southwestern England.

‘We discovered that bumble-bees can use a combin
News Articles and Discussion   bio_man   1057   Asked 13 years ago
9. Blood flow in the kidneys starts in the ___________________ _ artery to the e
9. Blood flow in the kidneys starts in the ___________________ _ artery to the e
9. Blood flow in the kidneys starts in the ___________________ _ artery to the entire kidney. Blood flow
to each nephron is through the ________________ _____________ ; the ball of capillaries in the
nephron is the ___________________ _, and the ___________________ _ arteriole drains the nephron.
The ___________________ _ __________ drains blood from the kidney.
Anatomy and Physiology   Lissa   2851   Asked 12 years ago
A $290,000 issue of five-year bonds redeemable at par offers 9.21% coupons payable semi-annually. Wh
A $290,000 issue of five-year bonds redeemable at par offers 9.21% coupons payable semi-annually. Wh
A $290,000 issue of five-year bonds redeemable at par offers 9.21% coupons payable semi-annually. What is the premium or discount and the purchase price of the bonds to yield 8.2% compounded quarterly?
(a) The premium is ____
(Round the final answer to the nearest cent as needed. Round all intermediate values to six decimal places as needed.)
(b) The purchase price of
Mathematics   Anonymous   208   Asked 8 months ago
A 10.0 kg mass and a 19.5 kg mass are hung from either end of a bar of negligible weight. If the 10.
A 10.0 kg mass and a 19.5 kg mass are hung from either end of a bar of negligible weight. If the 10.
A 10.0 kg mass and a 19.5 kg mass are hung from either end of a bar of negligible weight. If the 10.0 kg is increased to 12.0 kg, the balance point shifts by 16.2 cm. How long is the bar?
Algebra   presidente   211   Asked 5 years ago
A 15-kg rocket is traveling horizontally at 40 m/s. The rocket explodes, breaking into two pieces.
A 15-kg rocket is traveling horizontally at 40 m/s. The rocket explodes, breaking into two pieces.
A 15-kg rocket is traveling horizontally at 40 m/s. The rocket explodes, breaking into two pieces. The 5-kg piece is traveling at 60 m/s in the same direction as the original intact rocket. What is the velocity of the other piece?

(1) 30 m/s in the same direction as the intact rocket
(2) 30 m/s in the opposite direction as the intact rocket
(3) 40 m/s in the sam
Physics   cloveb   139   Asked 6 years ago
A 157-lb stone falls from rest from a cliff. If the air resistance is proportional to the square
A 157-lb stone falls from rest from a cliff. If the air resistance is proportional to the square
A 157-lb stone falls from rest from a cliff.  If the air resistance is proportional to the square of the stone's speed, and the limiting speed of the stone is 125 ft/s, Find the differential equation of motion.
Mathematics   mathinator   185   Asked 6 years ago
A 2-kg mass sliding at 5 m/s collides elastically with a 3-kg mass sliding at 2 m/s as shown in
A 2-kg mass sliding at 5 m/s collides elastically with a 3-kg mass sliding at 2 m/s as shown in
A 2-kg mass sliding at 5 m/s collides elastically with a 3-kg mass sliding at 2 m/s as shown in the diagram. The collision is in a straight line, so it can be considered to me a one-dimensional collision. What is the speed of the 3-kg mass after the collision?

(1) 4.4 m/s (2) 1.4 m/s (3) 3.2 m/s (4) 5.0 m/s

PHY2053, Summer 2012 Exam
Physics   cloveb   172   Asked 6 years ago
A 2.00-lb weight hangs motionless from a spring and is seen to stretch the spring 8.00 in. from
A 2.00-lb weight hangs motionless from a spring and is seen to stretch the spring 8.00 in. from
A 2.00-lb weight hangs motionless from a spring and is seen to stretch the spring 8.00 in. from its free length.  It is pulled down an additional 1.00 in. and released.  Write the equation of motion of the weight, taking zero at the original (motionless) position.

a) x = sin 0.433t
b) x = cos 6.95t
c) x = cos 0.433t
d) x = sin 6.95t

Tha
Calculus   HOTmike   226   Asked 6 years ago
A 2011 survey conducted for the Canadian Media Research Consortium found that 81 percent of those ...
A 2011 survey conducted for the Canadian Media Research Consortium found that 81 percent of those ...
[html]A 2011 survey conducted for the Canadian Media Research Consortium found that 81 percent of those surveyed would refuse to pay for access if their favourite online news sites erected a pay wall. Rather, they would simply go to sites where they could get their news for free. What impact might this have o
Sociology   RichaDuggi13   203   Asked A year ago
A 3-kg uniform disk has a radius of 0.6 m. An additional 1-kg mass is glued to the edge of the disk.
A 3-kg uniform disk has a radius of 0.6 m. An additional 1-kg mass is glued to the edge of the disk.
A 3-kg uniform disk has a radius of 0.6 m. An additional 1-kg mass is glued to the edge of the disk. What is the rotational inertia of the combination if the disk/mass combination rotates about the center of the disk?

(1) 0.90 kg-m2 (2) 0.54 kg-m2 (3) 0.36 kg-m2 (4) 0.72 kg-m2 (5) None of these.

PHY2053, Summer 2012 Exam
Physics   cloveb   152   Asked 6 years ago
A 30 year old man, a mechanic by profession came to the emergency department complaining of generali
A 30 year old man, a mechanic by profession came to the emergency department complaining of generali
A 30 year old man, a mechanic by profession came to the emergency department complaining of generalized body rigidity of two days duration. He has no Hx of fever, shortness of breath or cough.
On physical examination Vital signs are with in normal limit.
He is in opisthothonic position, he has torticolis and the deep tendon reflexes are exaggerated.
He is also foun
Anatomy and Physiology   mary   1154   Asked 13 years ago
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