Upper-Year Courses

Can pGEM-T Easy Vector JM109 cells take up multiple copies of the Plasmid?

Can pGEM-T Easy Vector JM109 cells take up multiple copies of the Plasmid?

I am using JM109 cells and the pGEM-T easy vector system II to clone a mixed microbial community for sequencing and I need to know whether a sinle copy or multiple copies can be taken up by each competent cell because this will impact on my ability t

!JRon@n   873   N/A

!JRon@n

Lab

Lab

Why is it important to incubate the inoculated plates at 37 degrees C

1LovelyNurse   902   2 Replies

duddy

Biotechnology - chapter 20

Biotechnology - chapter 20

whats the differences between bacteria express system and eukaryotic cloning and express system?

abertista   1917   1 Reply

habiba

PhysioEx Help?

PhysioEx Help?

Hate PhysioEx and need help!

acushman   1548   3 Replies

bio_man

Microbioloby case study question: Gram-positive, endospore-forming bacillius

Microbioloby case study question: Gram-positive, endospore-forming bacillius

B. Douglas is admitted to the hospital to receive IV antibiotics for treatment of folliculitis.  He began a second round of antibiotics a few days ago, this time ampicillin. Doug has had diarrhea, abdominal cramps and fever.  Blood tests re

adpeckham   758   1 Reply

padre

PhysioEX exercise 3 Help?

PhysioEX exercise 3 Help?

Anyone know the answers to exercise 3, activities 6, 7 and 8?

12

adr1e   17810   21 Replies

alymag912

PhysioEx Exercise 3 - Review

PhysioEx Exercise 3 - Review

This probably makes me seem lazy but does anyone have the answers to the Review for Exercise 3. I have a physio lecture exam to study for as well as a anatomy exam. -___________- I started the review I finished the matching aw well as question 16. An

adr1e   3931   3 Replies

adr1e

Why dilute ligation mixture before transformation?

Why dilute ligation mixture before transformation?

I understand that diluting the ligation reaction mixture prior to transformation increases the transformation efficiency, but why is it that less DNA yields better results in this case?

adriata   487   2 Replies

psyche360

How can you design an experiment to show segregation of proteins in rafts?

How can you design an experiment to show segregation of proteins in rafts?

This can be in a live or artificial cell

aefinan   1987   1 Reply

padre

Incubators (Contaminated vs Non contaminated)

Incubators (Contaminated vs Non contaminated)

Hello,

 

I have a question regarding incubators. Where I work, we segregate our so called contaminated work (intentionaly contaminated media/agar plates) and non contaminated work (product analysis wich are usualy exempt of bact

Aenhim   564   1 Reply

padre

Frog cardiovascular Exercise 6 in PhysioEx 8

Frog cardiovascular Exercise 6 in PhysioEx 8

I need help with frog cardiovascular exercise 6 in physio ex 8.0 =( help please!!!

afields5   3116   4 Replies

bio_man

PHYSio

PHYSio

Which of the following situations delay the development of muscle fatigue the longest?

Can anyone help on this please.

aggie25101   732   1 Reply

nite_n_day

Microbiology

Microbiology

I did a kirby-baur test or a disk diffusion method test, this evaluates the efficiency of a antimicrobial/bactericidal/disinfectant agent, by placing disks soaked in said antimicrobials on a agar that has been inoculated with a specific bacteria. A z

AJ   2369   2 Replies

natali123

PhysioEx 8.0 Exercise 3 Activity 6 and 7

PhysioEx 8.0 Exercise 3 Activity 6 and 7

Hello!  I need help with PhysioEx 8.0 Exercise 3 Activities 6 and 7.  The assignment is due Friday!  Can someone please help me?  Thanks in advance!

alaskatree   10520   4 Replies

star

ACCT410 Questions

ACCT410 Questions

E2-3   (Fund and Nonfund Accounts Identification) Indicate the fund or nonfund accounts that should be used to account for each of the following:
1.   Tax revenues restricted for road maintenance. Special Revenue Funds
2.   Resources restricted f

amashlee88   15036   2 Replies

Janie73004

Would you please write a discussion on this carbon NMR and identify the signals.

Would you please write a discussion on this carbon NMR and identify the signals.

I know the structure of product is D- Glucose and i need to explain every signal and peak. Please write a discussion about this Spectrum that related to D- glucose.
This specturm belong to my Lab report,please explain everything on detail that h

Amin.noorian   1690   3 Replies

duddy

please write a full discussion in this H Nmr and explain each signal

please write a full discussion in this H Nmr and explain each signal

I need help on H NMR ,i have to writea discussion on this specturm,I know the structure of product is D- glucose, but i could not write a discussion on it. Please explain everythingabout theses spectrum and how each signal related to the D- Glucose.

Amin.noorian   1230   N/A

Amin.noorian

The Endocrine System

The Endocrine System

The hypothalamus is involved in the release of epinephrine from the adrenal medulla through neurons of the:
a) somatosensory nervous system
b) parasympathetic nervous system
c ) sympathetic nervous system
d) central nervous system

amin19   3279   2 Replies

kapsara

At what pH did you see the highest activity of salivary amylase? Why?

At what pH did you see the highest activity of salivary amylase? Why?

The following questions refer to Activity 1: Assessing Starch Digestion by Salivary Amylase.
1. At what pH did you see the highest activity of salivary amylase? Why?
2. How do you know that the amylase did not have any contaminating malto

AMIRA11   5295   4 Replies

duddy

How to use STEREOLOGY (optical fractionator) in image stacks (confocal microsc.)

How to use STEREOLOGY (optical fractionator) in image stacks (confocal microsc.)

Hi! I'm using stack images after confocal microscopy to measure 2 types of cells in mouse brain after status epilepticus. PV cells (red channel) and VVA cells (green channel). I also want to measure their co-localization. Which is the best way t

anikapl   1410   1 Reply

kconnors

Expressing two different membrane proteins on the same promoter?

Expressing two different membrane proteins on the same promoter?

How could I generate a stable mammalian cell line that expresses two different membrane proteins on the same promoter? I want to know what strategies I could take.

anonyvous   1074   1 Reply

dtimmons95

physioex8.0 lab 3 review sheet question 3.

physioex8.0 lab 3 review sheet question 3.

What was the effect of increasing voltage? How does this change correlate to changes in the nerve?

I know that the voltage is increased so action potential occurs, but don't know the second question

ansells09   1151   2 Replies

ansells09

Physio ex 9.0 Activity 5 exercise 1

Physio ex 9.0 Activity 5 exercise 1

2.) Explain why there was no sodium transport even though ATP was present?


3.) Explain why the addition of glucose cariers had no effect on sodium of potassium transport?

ansocegueda   3452   5 Replies

Dove2011

Is anyone kind enough to share the PHYSIOEX 9.0 answers?

Is anyone kind enough to share the PHYSIOEX 9.0 answers?

I don't have my physioex 9.0 cd because my classmate left it at home when i needed it today, so he couldnt give it to me. Tomorrow is the due date and i was assigned to answer EXERCISE 2 ACTIVITY 6, all i need are the values for the graphs. Plea

aobg   1726   2 Replies

Alyyyyyyy

Delay Artefacts In Nerve Conduction Velocity

Delay Artefacts In Nerve Conduction Velocity

I was shown a demonstration on estimating peripheral nerve conduction velocity in a healthy subject and have been asked about to look up about delay artefacts which limits the accuracy of the values. There is apparently two of these but I have not be

Archer502   502   N/A

Archer502

Microbiology unknown help

Microbiology unknown help

17 possible tests i can run. I don't know where to start with the "flow of things". say if i get staph, what tests should I run to differentiate between the others? Is there an outli

asdzxc   465   1 Reply

padre

Can someone explain the AGID assay to me?

Can someone explain the AGID assay to me?

I am researching information for veterinarians on avian influenza and I am not clear on why you would perform an AGID instead of an ELISA.  I guess I do not really understand what the AGID test is and why it is optimal for this disease.
Hmmm, so why

Asinense   572   2 Replies

les4212

How do you interpret the spots in a CAT enzyme assay?

How do you interpret the spots in a CAT enzyme assay?

How do you interpret the spots in a CAT enzyme assay?

Asinense   617   1 Reply

les58mac

How do you simply explain cell proliferation assay(MTT)?

How do you simply explain cell proliferation assay(MTT)?

I am working on whitebloodcell proliferation. how can i relate this to MTT? what criteria to use? Im confused

Asinense   587   2 Replies

irina

in a fire assay for gold what is the best way to overcome the effect of ilmenite and iron in the crucible?

in a fire assay for gold what is the best way to overcome the effect of ilmenite and iron in the crucible?

in a fire assay for gold what is the best way to overcome the effect of ilmenite and iron in the crucible?

asing96   535   1 Reply

smokebomb13

how can i calculate the molarity of Ammonia solution with a minimum assay of 25% and a molecular weight of 17?

how can i calculate the molarity of Ammonia solution with a minimum assay of 25% and a molecular weight of 17?

using the following reaction, am bit lost on how to calculate the molarity of NH4OH solution:
AL2(SO4)3+ 6 NH4OH = 2 AL(OH)3+ 3(NH4)2SO4
will greatly appreciate your help on how to calculate the molarity of the Solution to start working on the reacti

asing96   4316   2 Replies

Illuminatus

what is yeast two-hybrid analysis and what is an overlay assay and what is a dot overlay assay?

what is yeast two-hybrid analysis and what is an overlay assay and what is a dot overlay assay?

please answer the whole question. thanks.

asinger96   712   1 Reply

smokebomb13

What are the most important controls for a CHIP assay?

What are the most important controls for a CHIP assay?

What are the most important controls for a CHIP assay?

asinger96   500   1 Reply

les4212

In microbiology, what does 'spoVM::spc' signify?

In microbiology, what does 'spoVM::spc' signify?

what does 'spoVM::spc' signify??
thanks:)

Asmaa   565   2 Replies

Fire_Fly

Laboratory assistant ettiquette?

Laboratory assistant ettiquette?

Hi,

So I'm not sure if this is the correct place to post this, but I thought I'd try.  I'm in my junior year of undergrad, majoring in biology and I recently began working in a research laboratory.  This lab does n

atg   1989   3 Replies

how_mendel

Can someone help with Exercise 3 Activitys 6-9?

Can someone help with Exercise 3 Activitys 6-9?

I have been working on Exercise 3 for a while now and I first of all do know how to get these answers for the charts/tables and I also dk know how to know if they are right. If someone does not mind helping me out with the tables for Activities 6-9 i

awhitley001   1217   1 Reply

charlesisfat

Preparig Histological Sections

Preparig Histological Sections

When we are preparing histological sections,
Are the Zenker & Bouin fixatives?
and if they are fixatives , are they complex fixatives ?

Aya-AlShreief   894   3 Replies

bio_man

Membrane potential always same value between cell body & axon?

Membrane potential always same value between cell body & axon?

When a neuron is placed in an ECF solution of varying concentrations (i.e., high K+, high Na+, etc.) and then you measure the membrane potential first of intracellular cell body and then measure the membrane potential in the intracellular axon, they

ayh1022   1888   2 Replies

Andre

Specificity of linkages in Deoxy ribose and ribose sugar?

Specificity of linkages in Deoxy ribose and ribose sugar?

hi,
In Deoxy ribose sugar -OH group is present in 5' , 3' ,1' , so there are 3 propabillity for dehydration reaction with a phosporic acid. but phosphate is bound to 5' and 3' carbon alone. why?

Then why deoxy

barathvaj   759   1 Reply

mikael

how to dissolve uric acid?

how to dissolve uric acid?

Hi,
I want to dissolve uric acid ?. I need to dissolve uric acid for isolating uricase producing bacteria or uric acid utilising bacteria. when i prepare nutrient media with uric acid, it's getting sediment-ed down in conical flask...how to

barathvaj   3455   1 Reply

bio_man