Upper-Year Courses

Delay Artefacts In Nerve Conduction Velocity

Delay Artefacts In Nerve Conduction Velocity

I was shown a demonstration on estimating peripheral nerve conduction velocity in a healthy subject and have been asked about to look up about delay artefacts which limits the accuracy of the values. There is apparently two of these but I have not be

Archer502   502   N/A

Archer502

(surface plasmon resonance) which method are helpful for reduce negative curve?

(surface plasmon resonance) which method are helpful for reduce negative curve?

1X tbs w/ sample
(1X TBS buffer : 90% DW+10% 10X TBS)

<1>
sample 100ul(diluted at DW until 1000X)
DW 800ul
10X TBS 100ul

it's my co-worker's opinion. he said "sample in DW, then when you

Koreanraichu   772   N/A

Koreanraichu

During chlorophyll biosynthesis, which wavelength of light, if at all, would produce the optimum ...

During chlorophyll biosynthesis, which wavelength of light, if at all, would produce the optimum ...

I am performing an experiment of chlorophyll biosynthesis, so had a question about whether during the reduction of the D ring by NADPH in chlorophyll biosynthesis, is the process affected by different wavelengths of light. It will also be very helpfu

jashdhill   1040   N/A

jashdhill

-20 microbial glycerol stocks

-20 microbial glycerol stocks

hello,

I am keeping microbial stocks in 25% glycerol at -20 (-80 not available) for a few months. but I am noticing that the stocks are remaining liquid. will this affect the viability of the cells ? if so what can I do without repeating t

tahah   1328   N/A

tahah

Micronucleus testing, stain too dark?

Micronucleus testing, stain too dark?

Hello there!  First time posting. 

In any case, we did a micronucleus test on rat bone marrow in class this past week.  I followed the directions to a T, and timed my staining to the second, with my slide in the Wright st

Wifey2myWifey   830   N/A

Wifey2myWifey

Why heat can not reverse my formaldehyde cross-linking product

Why heat can not reverse my formaldehyde cross-linking product

I was trying to pull down some associated proteins with my bait protein by formaldehyde cross-linking. my bait protein is 3XFLAG-tagged. So my final step is to elute my proteins out by Low pH glycine. Then, the eluted protein was split into two sampl

wuchenggang   1513   N/A

wuchenggang

Testing Cancer Cells ?

Testing Cancer Cells ?

Hi,

I would like to test cancer cells. Practical Tests.
I can imagine to run some tests from cancer cells of a mouse.
Isolating them in a petri dish and testing different substances and reactions on them.

Are

remoz   904   N/A

remoz

How does the electronegativity of a metal affect the activity of catalase?

How does the electronegativity of a metal affect the activity of catalase?

   Without any further ado, I have a problem concerning my lab experiment. The research question about the topic of enzymes with which I came up (the shortened version of it is the title) is the one that I found the most amusing and interesting. So I w

Malefactor   1079   N/A

Malefactor

What does it mean by induced/uninduced cultures?

What does it mean by induced/uninduced cultures?

When growing recombinant proteins I used a uninduced control culture and a auto induction medium in my production culture.

what does it mean by induced/uninduced

GCUQMU   1303   N/A

GCUQMU

Difficulty to come to a cunclusion from my SDS-PAGE gel electrophoresis results.

Difficulty to come to a cunclusion from my SDS-PAGE gel electrophoresis results.

In class I did a sds-page electrophoresis of e.coli expressed in four different sugars; Frucose, Arabinose, Glucose and Galactose, all in a concentration of 30 µl. When looking at my sds-page result, the four different samples seem to express the sam

Woahscience   2611   N/A

Woahscience

please write a full discussion in this H Nmr and explain each signal

please write a full discussion in this H Nmr and explain each signal

I need help on H NMR ,i have to writea discussion on this specturm,I know the structure of product is D- glucose, but i could not write a discussion on it. Please explain everythingabout theses spectrum and how each signal related to the D- Glucose.

Amin.noorian   1231   N/A

Amin.noorian

How to calculate the stock material in PCR?

How to calculate the stock material in PCR?

Hi! I have a problem with filling this PCR table. Can anyone help me, please?

notimp   3432   N/A

notimp

Expression data - how to deal with it?

Expression data - how to deal with it?

Hello dear forum
I am working currently with the expression analysis of various genes by Affymetrix microarray chips. Here I come to terms with which I unfortunately can not do much.

In one illustration the absolute expression is spec

Jaccy   4551   N/A

Jaccy

Design an experiment outlining your techniques that will analyze the temporal expression pattern of

Design an experiment outlining your techniques that will analyze the temporal expression pattern of

Design an experiment outlining your techniques that will analyze the temporal expression pattern of a gene (gene X - mRNA expression) and its protein localization during gastrulation in three different organisms.
Please state the organisms you a

gkor29   3127   N/A

gkor29

TTEST calculations help!

TTEST calculations help!

Hi there, I had generated a knockdown cell pool using shRNA against my target gene and control. i then subject these cells to the same conditions (DNA damage, etc).

I run experiments in triplicate usually, and then generate them independen

mm123an   2366   N/A

mm123an

Formulation of a questionnaire based on Birth Order and Coping with Stress

Formulation of a questionnaire based on Birth Order and Coping with Stress

Pleasant Good Day to All,
I am doing my Thesis on the topic Birth Order and Coping. I am in the process of trying to put a questionnaire together that helps match my independent and dependant variables: Ordinal Position, Archetypical Profile, D

Rhyanbeau   822   N/A

Rhyanbeau

Calculating GFR

Calculating GFR

I need to calculate GFR using creatinine clearance and MDRD equation. All the data I have is two absorbances for a standard (start = .321) (stop = .358) and two absorbances for urine (start = .288) (stop = .456).  The urine volume was 230 mL.&nb

guitargirl0812   1046   N/A

guitargirl0812

Can pGEM-T Easy Vector JM109 cells take up multiple copies of the Plasmid?

Can pGEM-T Easy Vector JM109 cells take up multiple copies of the Plasmid?

I am using JM109 cells and the pGEM-T easy vector system II to clone a mixed microbial community for sequencing and I need to know whether a sinle copy or multiple copies can be taken up by each competent cell because this will impact on my ability t

!JRon@n   873   N/A

!JRon@n

what would cause the different migration rate between each wells in agarose gel electrophoresis?

what would cause the different migration rate between each wells in agarose gel electrophoresis?

I have been having this trouble. DNA sample travels at different rates between different wells but I know they have the same size. The wells toward the middle of the gel always travel slower and the bands across the gel look like a rainbow shape. It

flowe12   1455   N/A

flowe12

Molecular biology of the cell study guides

Molecular biology of the cell study guides

Does anyone know where i could find study guides to Molecular biology of the cell by alberts, et al?

forsheyc1   2360   N/A

forsheyc1

Help with Exercise 3 Neurophysiology of Nerve Impulses please?

Help with Exercise 3 Neurophysiology of Nerve Impulses please?

Activity 2 Q 3. Identify which of the stimulus modalities induced the largest amplitude receptor potential in the Pacinian corpuscle. How well did the results compare with your prediction?

A2 Q4. Identify which of the stimulus modalities

HollyEV   7035   N/A

HollyEV

Human Anatomy Exercises Post 6

Human Anatomy Exercises Post 6

I am posting some of my completed exercises from the lab manual Human Anatomy Laboratory Manual with Cat Dissections (6th ed.) by Elaine Marieb and Susan J. Mitchell. I will warn you ahead of time some of the s

zanydelight69   1369   N/A

zanydelight69

In the experiment, explain what happened to active force as the ‘voltage’ increased? As it

In the experiment, explain what happened to active force as the ‘voltage’ increased? As it

Hello there!
  I just completed activities 6-8 in exercise 2 of the Phsyio Ex 8.0 book, and my professor wants me to to be able to answer some questions related to the experiments.  I answered most of them, but I'm not entirely su

Kate   7049   N/A

Kate

PhysioEx 18B Solutions

PhysioEx 18B Solutions

Hey there, does anyone have the solutions to PhysioEx 18B Neurophysiology of Nerve Impulses? I've done the questions but I wanna check them over before I hand it in so I can make sure I do well on it. It's from the A&P lab manual. Thank

ErikIsAHuman   2588   N/A

ErikIsAHuman

PhysioEx 8.0 exercise 4

PhysioEx 8.0 exercise 4

Looking for key to exercise 4 activity 5-8.  Must grade papers for instructor tonight, thank you for any help possible.

tigger too!   1941   N/A

tigger too!

What is the relationship between nerve size and conduction velocity?

What is the relationship between nerve size and conduction velocity?

I have finished the lab but am stuck on the last 3 questions in the activity 8.

5. What is the relationship between nerve size and conduction velocity?  What are the physiological reasons for this relationship?
I kno

havoc-covah   2524   N/A

havoc-covah

Describe your four tracings and explain what has happened in each of them.

Describe your four tracings and explain what has happened in each of them.

I am stuck on just a few questions and wondered if anyone could help me out.

Activity 3 question: An individual muscle fiber follows the all-or-none principle—it will either contract 100% or not at all.  Does the muscle we are working

sunshine   1598   N/A

sunshine