Biochemistry

Western blot help

Western blot help

Hi guys, I’ve got an exam and we’ve been given this beforehand. What kinda question do you think will be asked on this and any answers would be appreciated.

Excerpt

Figure A shows a silver stained isoelectric focusing gel of

Anonymous   33   1 Reply

duddy

Calculate the number of times a recognition site for a 4 bp restriction endonuclease is likely to ...

Calculate the number of times a recognition site for a 4 bp restriction endonuclease is likely to ...

Calculate the number of times a recognition site for a 4 bp restriction endonuclease is likely to occur in the diploid human genome

alpsi   120   2 Replies

Anonymous

Locate the H+ and OH- ions from the water molecule

Locate the H+ and OH- ions from the water molecule

locate the H+ and OH- ions from the water molecule that was split (hydrolyzed) in this reaction, showing esculin hydrolysis.

last problem on the attachment #4 not sure about this one..

[img width=350 height=132]https://biology-

12

bronze   10843   20 Replies

Duong!

An unknown amount of He gas occupies 30.5 L at 2.00 atm pressure and 300 K. What is the weight of ...

An unknown amount of He gas occupies 30.5 L at 2.00 atm pressure and 300 K. What is the weight of ...

An unknown amount of He gas occupies 30.5 L at 2.00 atm pressure and 300 K. What is the weight of the gas in the container?
 a. 2.48 g c. 9.91 g
  b. 4.95 g d. 19.8 g

Abessesen   304   3 Replies

MSROGERS

Eukaryotic chromatin replication

Eukaryotic chromatin replication

Eukaryotic chromatin replication:
A.  Is facilitated by the presence of a large pool of histones which are synthesized uniformly throughout the cell cycle.
B.  Is characterized by conservative segregation of pre_existing H3_H4 tet

couple007   409   1 Reply

bolbol

Proteosomes are multifunctional, multisubmit protein

Proteosomes are multifunctional, multisubmit protein

Proteosomes are multifunctional, multisubmit protein complexes that
A.  convert amino acids to glucose and ketone bodies during starvation.
B.  degrade most cellular proteins to their consistent amino acids.
C.  facilita

couple007   321   1 Reply

bolbol

The formula of a particular compound can be written as (i) C4H10 (ii) CH3CH2CH2CH3 (iii) ...

The formula of a particular compound can be written as (i) C4H10 (ii) CH3CH2CH2CH3 (iii) ...

The formula of a particular compound can be written as (i) C4H10 (ii) CH3CH2CH2CH3 (iii) CH3(CH2)2CH3 Which of the following is true?
 a. All of these provide exactly the same amount of information.
  b. Formula (ii) provides more informat

Angela26kristin   229   3 Replies

jeooncdaoc

Estimate the pH of the resulting solution prepared by mixing 1.0 mole of solid disodium phosphate ...

Estimate the pH of the resulting solution prepared by mixing 1.0 mole of solid disodium phosphate ...

Estimate the pH of the resulting solution prepared by mixing 1.0 mole of solid disodium phosphate (Na2HPO4) and 1.25 mole of hydrochloric acid. The pKa values for phosphoric acid are 2.1, 7.2, 12.4.
 
  a. pH < 2.1
  b. pH = 2.1

Random   160   2 Replies

Anonymous

Clostridium Sporogenes not growing in nutrient broth

Clostridium Sporogenes not growing in nutrient broth

I'm working with a student who cannot grow Clostridium sporogenes in nutrient broth incubated at 37C (over 6 hrs). Is he missing anything, PH, any key ingredient, etc?
note: Bacteria was freshly acquired

GSan T   106   1 Reply

bio_man

Chair conformation and stability

Chair conformation and stability

A)  Use the provided structures of D-mannose and L-mannose and complete the structures
of these two sugars in chair conformation by drawing the remaining hydroxyl (OH)
groups at the correct positions (axial or equatorial). Predict t

Anonymous   301   2 Replies

Anonymous

Chair Conformations

Chair Conformations

Draw all of the possible chair conformations, identify cis and trans conformations, and arrange the order of stability for these conformations.


A) 1,3-dihydroxycyclohexane



B) 1,4-dibromocyclohexane

Anonymous   199   2 Replies

Anonymous

When preparing an acetate buffer at pH 4.5 with 0.01 M solutions of acetic acid (pKa = 4.8) and ...

When preparing an acetate buffer at pH 4.5 with 0.01 M solutions of acetic acid (pKa = 4.8) and ...

When preparing an acetate buffer at pH 4.5 with 0.01 M solutions of acetic acid (pKa = 4.8) and sodium acetate, the volume of acetic acid needed would be ____ the volume of sodium acetate solution.
 
  a. equal to
  b. less than half

mahi   286   3 Replies

Lettyyys

Procedure to identify protein markers for pathology diagnosis

Procedure to identify protein markers for pathology diagnosis

I'm a biochemistry student and I'm stuck on this question:Propose a procedure to identify protein markers for pathology diagnosis ?

Chou Rar   117   1 Reply

Anonymous

Resonance structures

Resonance structures

Provide all possible resonance structures for the following compound.

Anonymous   122   1 Reply

Anonymous

Lewis Base and Acid

Lewis Base and Acid

Using the listed compounds, note what compounds are able to function as a Lewis Base and/or a Lewis Acid.

NH3

CO32-

Cl-

H[

Anonymous   98   1 Reply

Anonymous

Electron-pushing mechanism for acid-base reactions

Electron-pushing mechanism for acid-base reactions

Use the Electron-Pushing mechanism to illustrate and complete the following acid-base reactions. Specify what the acid, base, conjugate acid, conjugate base, and what compound is donating the proton

A)  HCO3-[/s

Anonymous   100   1 Reply

Anonymous

Hybridization

Hybridization

Determine the hybridization of atomic orbital for the carbon and oxygen atoms in the following compound (use the given table and numbering for the specification of atoms). What are the bond angles between C1-C2-C3 and C3-C4-05? What kinds of orbitals

Anonymous   109   1 Reply

Anonymous

Define & contrast these 3 terms...

Define & contrast these 3 terms...

Fermentation,
Oxidative phosphorylation,
Aerobic resperation

Kabbces   9434   1 Reply

bolbol

Name and describe the three ways to add phosphate to ADP to make ATP

Name and describe the three ways to add phosphate to ADP to make ATP

Name & describe 3 ways to add phosphate to ADP to make ATP

Kabbces   818   1 Reply

bolbol

Calculate the pH of a 0.2 M acetate buffer (pKa = 4.77) that contains twice as much acid as ...

Calculate the pH of a 0.2 M acetate buffer (pKa = 4.77) that contains twice as much acid as ...

Calculate the pH of a 0.2 M acetate buffer (pKa = 4.77) that contains twice as much acid as conjugate base.

alpsi   1495   13 Replies

Anonymous

Reaction speed is linearly proportional to substarte concentration over the whole range of substrate ...

Reaction speed is linearly proportional to substarte concentration over the whole range of substrate ...

Reaction speed is linearly proportional to substarte concentration over the whole range of substrate concentrations

A) True
B) False

SkövdeUni   677   1 Reply

bolbol

An ammonia buffer contains NH3:NH4+ in a ratio of 0.4 moles:0.6 moles (pK = 9.75). What will be the ...

An ammonia buffer contains NH3:NH4+ in a ratio of 0.4 moles:0.6 moles (pK = 9.75). What will be the ...

An ammonia buffer contains NH3:NH4+ in a ratio of 0.4 moles:0.6 moles (pK = 9.75). What will be the pH if you add 0.01 moles of HCl to this buffer?
 A) 8.98
  B) 9.04
  C) 9.25
  D) 9.46
  E) 9.52

Angela26kristin   344   3 Replies

jianhong9991

What structural feature is most responsible for chlorophyll's ability to absorb

What structural feature is most responsible for chlorophyll's ability to absorb

What structural feature is most responsible for chlorophyll's ability to absorb light?
A) The magnesium ion.
B) The conjugated double-bonds in the tetrapyrrole ring.
C) The phytol side chain.
D) An aromatic side chain attache

mikael   412   1 Reply

bolbol

The main difference between photosynthesis and respiratory electron transport is

The main difference between photosynthesis and respiratory electron transport is

The main difference between photosynthesis and respiratory electron transport is (are)
A) the tetrapyrole ring of the pigments involved.
B) the coenzymes that carry electrons.
C) the sources of excited electrons.
D) the formation

mikael   415   1 Reply

bolbol

Chlorophyll molecules can exist in ________ different states.

Chlorophyll molecules can exist in ________ different states.

Chlorophyll molecules can exist in ________ different states.
A) 2
B) 3
C) 4
D) 5

mikael   440   1 Reply

bolbol

Photosynthesis by green sulfur bacteria and cyanobacteria are different because

Photosynthesis by green sulfur bacteria and cyanobacteria are different because

Photosynthesis by green sulfur bacteria and cyanobacteria are different because green sulfur bacteria ________.
A) have only cyclic photosynthesis
B) use photosystem I, but not photosystem II
C) use both photosystems I and II
D) u

mikael   331   1 Reply

bolbol

Which use only type I reaction centers for photosynthesis?

Which use only type I reaction centers for photosynthesis?

Which use only type I reaction centers for photosynthesis?
A) Heliobacteria.
B) Purple bacteria.
C) Cyanobacteria.
D) Algae.

mikael   273   1 Reply

bolbol

What family of genes are frequently carried by bacterial transposons

What family of genes are frequently carried by bacterial transposons

What family of genes are frequently carried by bacterial transposons?
A.  Antibiotic resistance
B.  F factor
C.  Origins of replication
D.  Restriction endonucleases
E.  Toxin production

couple007   567   1 Reply

bolbol

Photosynthesis in PS I is non-cyclic because

Photosynthesis in PS I is non-cyclic because

Photosynthesis in PS I is non-cyclic because
A) it is slower than those in PS II.
B) reaction centers must be reduced by electrons from another source.
C) reaction centers are more tightly packed in the photosystem complexes.
D)

mikael   657   1 Reply

bolbol

Reactions to extract electrons from water require ________ to reverse electron f

Reactions to extract electrons from water require ________ to reverse electron f

Reactions to extract electrons from water require ________ to reverse electron flow.
A) powerful oxidizing agents
B) powerful reducing agents
C) dark reactions
D) ATP

mikael   496   1 Reply

bolbol

Photon capture by plant chloroplasts is more efficient than in bacteria because

Photon capture by plant chloroplasts is more efficient than in bacteria because

Photon capture by plant chloroplasts is more efficient than in bacteria because they contain
A) both PS I and PS II.
B) more chlorophyll.
C) LHII, a light harvesting complex.
D) both cyclic and noncyclic photophosphorylation.

mikael   449   1 Reply

bolbol

The enzyme glycoprotein peptidase is irreversibly inhibited by penicillin, which forms a covalent ...

The enzyme glycoprotein peptidase is irreversibly inhibited by penicillin, which forms a covalent ...

The enzyme glycoprotein peptidase is irreversibly inhibited by penicillin, which forms a covalent adduct with a serine residue on the enzyme. From this observation which statement logically follows?
 a. Serine must be protonated for this enzyme

Greenspotsock   386   3 Replies

bio_man

Which of the following reactions IS NOT INVOLVED in regulation of the phosphoryl

Which of the following reactions IS NOT INVOLVED in regulation of the phosphoryl

Which of the following reactions IS NOT INVOLVED in regulation of the phosphorylase kinase cascade?
A.  formation of cyclic AMP.
B.  activation of protein kinase.
C.  phosphorylation of certain amino acids of phosphoryla

couple007   636   1 Reply

bolbol

Wavelengths of Light

Wavelengths of Light

   Dear Biochemistry Community:
     Could it be that the plant pigments in leaves help to filter out which wavelengths of  light are not necessary in plant metabolism?  As an example:  The green of chlorophyll filters out

StanleyJoe   173   2 Replies

Thomas Kistner

Question about bases of DNA

Question about bases of DNA

The question consists of a table in which the percentage of different bases of DNA molecule in a human are written like this:
Source of DNA.     %Adenine.      %Guanine.     %Thymine.     %Cytosine

Happyhuman001   258   3 Replies

Anonymous

Chemicals that bind to DNA by intercalating between

Chemicals that bind to DNA by intercalating between

Chemicals that bind to DNA by intercalating between the bases
A.  can cause frame shift mutations
B.  can cause deletion mutations
C.  can cause insertion mutations
D.  are generally planar hydrophobic molecule

couple007   453   1 Reply

bolbol

DNA As Polymer

DNA As Polymer

Dear Biochemistry Community:
     Could anyone think of the DNA molecule as a polymer, its constituent amino acids as monomers, and the RNA molecules as being organic
catalysts, that is, as enzymes?  Thank you.

StanleyJoe   126   1 Reply

bio_man

Complete the following reaction for the proteolytic cleavage

Complete the following reaction for the proteolytic cleavage

The activation of chymotrypsinogen, a zymogen, requires the cleavage of a bond between Arg-15 and Ile-16, and the subsequent attraction of Ile-16 and Asp-194. Complete parts (a) and (b). a) Complete the following reaction for the proteolytic cleavage

123

locklearl   23469   39 Replies

TacoSauce71

What is the product of the reaction catalyzed by rubisco in the Calvin cycle? A

What is the product of the reaction catalyzed by rubisco in the Calvin cycle? A

What is the product of the reaction catalyzed by rubisco in the Calvin cycle?
A) Dihydroxyacetone phosphate.
B) 3-phosphoglycerate.
C) Ribose 5-phosphate.
D) Fructose 6-phosphate.

mikael   376   1 Reply

bolbol

Attempts to genetically modify rubisco to help increase food production might in

Attempts to genetically modify rubisco to help increase food production might in

Attempts to genetically modify rubisco to help increase food production might include trying to ________.
A) enhance the carboxylation reaction and suppress the oxygenation reaction
B) suppress the carboxylation reaction and enhance the oxy

mikael   314   1 Reply

bolbol