Biochemistry

Both purines and pyrimidines can exist in different tautomeric forms. The forms typically found in ...

Both purines and pyrimidines can exist in different tautomeric forms. The forms typically found in ...

Both purines and pyrimidines can exist in different tautomeric forms. The forms typically found in double-stranded DNA are:
 a. uracil = keto; adenine = keto
 b. guanine = keto; cytosine = enol
 c. thymine = keto; guanine = keto

kescobar5   65   2 Replies

kescobar5

In the nucleotides found in nucleic acids, the linkage between the bases and the sugar involve:

In the nucleotides found in nucleic acids, the linkage between the bases and the sugar involve:

In the nucleotides found in nucleic acids, the linkage between the bases and the sugar involve:
 a. N-1 of pyrimidines and C-1 of the sugar
 b. N-3 of pyrimidines and C-1 of the sugar
 c. N-1 of purines and C-5 of the sugar
 d. N

ahh_risa   58   1 Reply

dalveixes

An important regulator of cellular metabolism is:

An important regulator of cellular metabolism is:

An important regulator of cellular metabolism is:
 a. 2'-3' cyclic TMP
 b. 2'-3' cyclic UMP
 c. 3'-5' cyclic AMP
 d. 3'-5' cyclic UMP
 e. 2'-5' cyclic CMP

ksultz   37   1 Reply

dylbt

The process of transcription in eukaryotes involves the synthesis of:

The process of transcription in eukaryotes involves the synthesis of:

The process of transcription in eukaryotes involves the synthesis of:
 a. an RNA copy of a DNA sequence by an RNA polymerase
 b. a DNA copy of an RNA sequence by an RNA polymerase
 c. an RNA copy of another RNA by an RNA polymerase

MB0727   50   1 Reply

ally

Transfer RNAs are involved in:

Transfer RNAs are involved in:

Transfer RNAs are involved in:
 a. carrying nucleotides to the ribosome for messenger RNA synthesis
 b. carrying amino acids to the ribosome for protein synthesis
 c. carrying amino acids to peroxisomes for degradation
 d. carryi

Brooke.catlin   55   1 Reply

garroghuu

There are fundamental chemical differences between DNA and RNA, such as:

There are fundamental chemical differences between DNA and RNA, such as:

There are fundamental chemical differences between DNA and RNA, such as:
 a. The nucleotides in RNA are linked by 3'-5' phosphodiester bridges while those in DNA involve 2'-5' bridges.
 b. The nucleotides in RNA include uracil, while DNA h

Larrhea   66   1 Reply

neontoast91

DNA is much more stable to alkaline hydrolysis than RNA because:

DNA is much more stable to alkaline hydrolysis than RNA because:

DNA is much more stable to alkaline hydrolysis than RNA because:
 a. DNA is usually protected by proteins, while RNA is not.
 b. There are many more RNA digesting enzymes present in cells than DNA digesting enzymes.
 c. The 2'-OH grou

Larrhea   36   2 Replies

Larrhea

The difference between the sugars in DNA and RNA is:

The difference between the sugars in DNA and RNA is:

The difference between the sugars in DNA and RNA is:
 a. In RNA the sugar residue is neuraminic acid, while the sugar residue in DNA is deoxy-neuraminic acid.
 b. In DNA the sugar residue is deoxy-adenine, whereas in RNA it is adenine.

Jnortontruvisio   23   1 Reply

P.liania

What are the two most important discoveries that enabled biochemists to start determining the ...

What are the two most important discoveries that enabled biochemists to start determining the ...

What are the two most important discoveries that enabled biochemists to start determining the primary structure of nucleic acid polymers?
 a. restriction endonucleases
 b. diisofluorophosphate
 c. polyacrylamide gel electrophoresis

nclariceb   27   1 Reply

megabyte7

The strand of DNA that is to be complemented by the DNA polymerase is referred to as the:

The strand of DNA that is to be complemented by the DNA polymerase is referred to as the:

The strand of DNA that is to be complemented by the DNA polymerase is referred to as the:
 a. primer
 b. template
 c. daughter strand
 d. autoradiograph
 e. guanosine

ksultz   40   1 Reply

garroghuu

What is the property of the chain-terminating nucleotides that enables them to terminate the ...

What is the property of the chain-terminating nucleotides that enables them to terminate the ...

What is the property of the chain-terminating nucleotides that enables them to terminate the polymerizing DNA chain?
 a. They are radioactive.
 b. They lack the nucleotide base.
 c. They lack a 3'-hydroxyl.
 d. They are fluoresce

Griffinw42   27   1 Reply

Harley0507

When DNA strands of different length separate on a polyacrylamide gel, which of following occurs? ...

When DNA strands of different length separate on a polyacrylamide gel, which of following occurs? ...

When DNA strands of different length separate on a polyacrylamide gel, which of following occurs?
 a. Larger fragments travel faster.
 b. The electric field causes bands to break.
 c. Smaller fragments travel faster.
 d. The poly

Miller123   47   1 Reply

goldenchild22

When doing a DNA sequencing reaction, what is the minimum number of parallel reactions needed to get ...

When doing a DNA sequencing reaction, what is the minimum number of parallel reactions needed to get ...

When doing a DNA sequencing reaction, what is the minimum number of parallel reactions needed to get a complete sequence?
 a. 1
 b. 2
 c. 4
 d. 5
 e. 8

Abessesen   52   2 Replies

Abessesen

How many hydrogen bonds are formed between the complementary bases G:C and A:T, respectively?

How many hydrogen bonds are formed between the complementary bases G:C and A:T, respectively?

How many hydrogen bonds are formed between the complementary bases G:C and A:T, respectively?
 a. 2,3
 b. 3,2
 c. 3,4
 d. 4,3
 e. 1,2

hawkins1346   35   1 Reply

sophiamendoza94

A DNA double helix is described as a(n):

A DNA double helix is described as a(n):

A DNA double helix is described as a(n):
 a. parallel double helix
 b. antiparallel double helix
 c. hydrogen-bonded double helix
 d. a and c
 e. b and c

Random   68   1 Reply

megabyte7

What are the most common conformational forms of DNA?

What are the most common conformational forms of DNA?

What are the most common conformational forms of DNA?
 a. a, b and z
 b. A, B, and Z
 c. parallel, antiparallel, and triple helical
 d. b and c
 e. none of the above

Jovaini   49   1 Reply

sandyb2

What are properties of Z-form DNA?

What are properties of Z-form DNA?

What are properties of Z-form DNA?
 a. The glycosidic bonds are syn.
 b. It is a left-handed helix.
 c. The glycosidic bonds are anti.
 d. a and b
 e. b and c

lacinb30   26   1 Reply

rayohn2010

Which of the following factors increase the Tm of double-stranded DNA?

Which of the following factors increase the Tm of double-stranded DNA?

Which of the following factors increase the Tm of double-stranded DNA?
 a. increasing relative A:T content and decreasing ionic strength of the solution
 b. increasing relative G:C content and increasing the ionic strength of the solution

alshernandez   25   1 Reply

ally

In tRNA, some unusual bases are found. Some examples of these are:

In tRNA, some unusual bases are found. Some examples of these are:

In tRNA, some unusual bases are found. Some examples of these are:
 a. pseudouridine
 b. inosine
 c. methylguanosine
 d. all of the above
 e. none of the above

Griffinw42   36   1 Reply

car2065596

What is the main function of the invariant nucleotides in tRNA?

What is the main function of the invariant nucleotides in tRNA?

What is the main function of the invariant nucleotides in tRNA?
 a. to recognize specific codons
 b. to create the correct 3-D fold
 c. to allow tRNA synthetase to recognize the tRNA
 d. all of the above
 e. none of the abov

kristina92293   39   1 Reply

garroghuu

In order to be able to isolate plasmids containing a foreign gene of interest, cloning vectors ...

In order to be able to isolate plasmids containing a foreign gene of interest, cloning vectors ...

In order to be able to isolate plasmids containing a foreign gene of interest, cloning vectors usually contain a selectable marker. These selectable markers are often:
 a. inverted repeats
 b. restriction sites
 c. origins of replicat

bronzeclock   67   1 Reply

hsprouse

While virtually any DNA sequence can be cloned into plasmids, one of the practical limitations is ...

While virtually any DNA sequence can be cloned into plasmids, one of the practical limitations is ...

While virtually any DNA sequence can be cloned into plasmids, one of the practical limitations is that:
 a. Only bacterial DNA can be cloned into bacterial plasmids.
 b. Only very large pieces of DNA can be inserted efficiently into plasmi

Angela26kristin   37   1 Reply

salmajed1

The term copy number as applied to plasmids refers to:

The term copy number as applied to plasmids refers to:

The term copy number as applied to plasmids refers to:
 a. the number of kilobases of foreign DNA inserted into the plasmid
 b. the number of copies of a particular plasmid found per bacterial cell
 c. the number of copies of a specif

Pooh bear   36   1 Reply

saldii43

CDNA libraries are constructed by:

CDNA libraries are constructed by:

cDNA libraries are constructed by:
 a. synthesizing DNA copies of foreign genes from plasmids
 b. using PCR to non-specifically amplify DNA sequences using universal primers
 c. synthesizing cDNA from purified cellular mRNA using reve

starrlite81   54   2 Replies

starrlite81

DNA ligases are used to:

DNA ligases are used to:

DNA ligases are used to:
 a. cleave double-stranded DNA at specific restriction sites
 b. join the ends of foreign DNA to the free ends of cleaved plasmid DNA
 c. cleave single-stranded segments of DNA fragments to yield blunt ends

yjuhen   42   1 Reply

winger785

Taq DNA polymerase (from Thermus aquaticus) is often used in PCR reactions. The advantage of using ...

Taq DNA polymerase (from Thermus aquaticus) is often used in PCR reactions. The advantage of using ...

Taq DNA polymerase (from Thermus aquaticus) is often used in PCR reactions. The advantage of using this polymerase, compared with most other bacterial DNA polymerases, is that:
 a. The Taq DNA polymerase is almost error free for DNA synthesis.

Revolver38   59   1 Reply

ABascomb

To express a eukaryotic protein in E. coli, the eukaryotic DNA must be cloned into a vector which in ...

To express a eukaryotic protein in E. coli, the eukaryotic DNA must be cloned into a vector which in ...

To express a eukaryotic protein in E. coli, the eukaryotic DNA must be cloned into a vector which in addition to this foreign DNA must also have:
 a. Hind III sites on either end
 b. a streptavidin affinity ligand
 c. a reporter gene

kescobar5   39   1 Reply

elizabethxo1

In Southern blots, specific sequences of DNA are identified by:

In Southern blots, specific sequences of DNA are identified by:

In Southern blots, specific sequences of DNA are identified by:
 a. annealing with a labeled single-stranded DNA probe
 b. annealing with a labeled single-stranded RNA
 c. binding to radioactively labeled antibodies
 d. transcrib

Jnortontruvisio   34   1 Reply

MSROGERS

In the reverse transcriptase-driven synthesis of cDNA, oligo(dT) primers are typically used. These ...

In the reverse transcriptase-driven synthesis of cDNA, oligo(dT) primers are typically used. These ...

In the reverse transcriptase-driven synthesis of cDNA, oligo(dT) primers are typically used. These (dT) oligomers can function as effective primers because they:
 a. can readily be sealed by DNA ligase
 b. are good substrates for the rever

alshernandez   57   1 Reply

hellokristi4

Phage T4 DNA ligase is used to:

Phage T4 DNA ligase is used to:

Phage T4 DNA ligase is used to:
 a. anneal the single-stranded sticky ends of two pieces of DNA
 b. provide selectable markers in plasmids
 c. covalently join the blunt ends of DNA molecules
 d. create tailor-made ends on cloning

MB0727   70   1 Reply

Nrcp1978

An ori sequence is a:

An ori sequence is a:

An ori sequence is a:
 a. cloning site
 b. chimeric construct
 c. site where restriction endonucleases attack
 d. site signaling where DNA replication can begin
 e. site for antibiotic resistance

jennjenn563   49   1 Reply

mwarner93

Shuttle vectors are used to:

Shuttle vectors are used to:

Shuttle vectors are used to:
 a. join the ends of foreign DNA
 b. create sticky-ended DNA cuts
 c. covalently join blunt-end DNA fragments
 d. propagate ampicillin-resistant colonies
 e. transfer genes between two different

kekebab001   57   1 Reply

Nrcp1978

When DNA is packaged into phage heads by the bacteriophage lambda packaging system, it must have a ...

When DNA is packaged into phage heads by the bacteriophage lambda packaging system, it must have a ...

When DNA is packaged into phage heads by the bacteriophage lambda packaging system, it must have a 14-bp sequence at each end of its ends. This sequence is known as:
 a. a cohesive end site
 b. cosmid vector
 c. shuttle vector
 d

Random   45   1 Reply

garroghuu

When a foreign piece of DNA is inserted into a plasmid antibiotic resistance gene, the host organism ...

When a foreign piece of DNA is inserted into a plasmid antibiotic resistance gene, the host organism ...

When a foreign piece of DNA is inserted into a plasmid antibiotic resistance gene, the host organism becomes sensitive to this antibiotic. This antibiotic sensitivity is used to selectively identify which plasmids contain the inserted gene of interes

bronzeclock   69   1 Reply

hennihanadi

Shot-gun cloning involves trying to:

Shot-gun cloning involves trying to:

Shot-gun cloning involves trying to:
 a. Clone all the genes of an organism at one time.
 b. Transform an organism by introducing foreign genes with a gene gun.
 c. Create a synthetic combinatorial library of all the genes of an organ

motee   63   2 Replies

motee

1 The turnover number of an enzyme is the number of:

1 The turnover number of an enzyme is the number of:

1 The turnover number of an enzyme is the number of:
 a. enzyme-substrate complexes formed per unit time
 b. substrate molecules converted to product before the enzyme is saturated
 c. substrate molecules converted into product per en

motee   66   1 Reply

garroghuu

In the presence of a pure non-competitive inhibitor:

In the presence of a pure non-competitive inhibitor:

In the presence of a pure non-competitive inhibitor:
 a. The Km is increased.
 b. The Km is decreased.
 c. The Vmax is increased.
 d. The Vmax is decreased.

hawkins1346   31   1 Reply

Beehappy2

If a plot of the reciprocal of the velocity of an enzyme catalyzed reaction versus the reciprocal of ...

If a plot of the reciprocal of the velocity of an enzyme catalyzed reaction versus the reciprocal of ...

If a plot of the reciprocal of the velocity of an enzyme catalyzed reaction versus the reciprocal of the substrate concentration yields a straight line, the intercept on the 1/V axis is:
 a. V/Km
 b. -1/Km
 c. Km/Vmax
 d. 1/Vmax

mahi   66   1 Reply

John Ross

For an enzyme reaction obeying Michaelis-Menten kinetics the horizontal part of a v versus S plot is ...

For an enzyme reaction obeying Michaelis-Menten kinetics the horizontal part of a v versus S plot is ...

For an enzyme reaction obeying Michaelis-Menten kinetics the horizontal part of a v versus S plot is indicative of:
 a. a first-order reaction with respect to substrate
 b. a second-order reaction with respect to substrate
 c. a mixed

crownlessking   51   1 Reply

hellokristi4

Malonate (-OOC-CH2-COO-) can depress operation of the tricarboxylic acid cycle by:

Malonate (-OOC-CH2-COO-) can depress operation of the tricarboxylic acid cycle by:

Malonate (-OOC-CH2-COO-) can depress operation of the tricarboxylic acid cycle by:
 a. competitively inhibiting succinate dehydrogenase
 b. complexing with an essential metal ion factor
 c. complexing with acetyl-CoA
 d. favoring

ahh_risa   45   1 Reply

elizabethxo1