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PR3CiSiON PR3CiSiON
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7 years ago Edited: 5 years ago, how_mendel
To confirm the semiconservative model of replication, it was important for Meselson and Stahl to quantify the amount of DNA in each band produced by density-gradient centrifugation. To accomplish this, they took advantage of the fact that DNA absorbs ultraviolet light, and used UV light to photograph each tube.
By scanning the UV photographs with a microdensitometer, graphs like the ones below were produced. The height of each peak in the graph is directly proportional to the concentration of DNA in the corresponding band. Also, the position of each peak reflects the 14N and 15N content of the band.
Suppose that the scientists analyzed the same amount of DNA (10 units) by density-gradient centrifugation after two, three, and four rounds of replication in 14N medium. What would you predict the microdensitometer graph would look like after each round?

Drag each microdensitometer graph to the appropriate bin.
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padrepadre
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Thank you!
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Thank you. This was helpful! This was a tricky one!!  Undecided
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merci!
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Wow! I never would have figured that out!
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