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klee95 klee95
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Posts: 6
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10 years ago
In the pUC plasmids, a polylinker region that includes multiple restriction sites is embedded in the lacZ component. If a sequence of interest is inserted into this region, what is the effect on lacZ?

It's fill-in-the-blank. I've tried insertional inactivation, but that was incorrect.
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Answer rejected by topic starter
wrote...
10 years ago
Gene knockout.
klee95 Author
wrote...
10 years ago
Nope, tried it and it was incorrect.
wrote...
10 years ago
You are right that it is insertional inactivaiton but try just "inactivation". I think that is the answer. Mastering Genetics is just dumb like that.
klee95 Author
wrote...
10 years ago
Well, I feel dumb now. Thank you!
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