1.)The odds of locating a specific 4 base sequence that a RE will recognize in a genome is:
a.)Once every 256 base pairs
b.)Once every 1024 base pairs
c.)Once every 4096 base pairs
D.)Twice every 1024 base pairs
e.)None of the above.
2.)Which one of the following is most likely to be a restriction enzyme recognition sequence? (Only one strand of a double stranded sequence of DNA is shown, in the 5'-3' direction)
a.)GTAATG
b.)AAATTT
c.)CAACAAG
d.)ATCAGA
e.)ATCGGT
3.)Which one of the following statements about plasmids is correct?
a.)They may carry genes that enable a bacteria to survive somewhere it would not be able to survive without a plasmid.
b.)They can only be transferred within a given bacterial species.
c.)Cells can only have one copy of a plasmid at any given time.
d.)They are required for bacterial survival even if resources are plentiful.
e.)It benefits a bacterial cell in the "wild" to have as many copies of a plasmid as possible (the higher the copy number, the better).
4.)If you cut organism A's DNA with BamHI and you cut organism B's DNA with the same enzyme, and then you mixed all the resulting DNA fragments together along with DNA ligase, what kind of DNA would you get back? If you predict that you could obtain the DNA sample listed, choose Yes. If you predict that the resulting DNA sample is not possible, choose No. (Assume that each organism's DNA would only contain one BamHI restriction site).
a.)Recombinant DNA from organisms A and B
b.)Religated DNA from organism B
c.)Religated DNA from organism A
Here what i think the answer are:
#1.)a
#2.)c
#3.)a
#4)i will say YES for all a,b,and c.
CORRECT me if i am WRONG.!!!!:)