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raquelwoods14 raquelwoods14
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6 years ago
You are given four additional polymeric materials (A, B, C, D) to examine for potential as a
  vascular graft material, and are told to use size-exclusion chromatography to determine the
  approximate molecular weights of the unknown polymers. You obtain the following
  information for the first three samples (A, B, C).
 
  a. Assuming monodisperse samples, what is the molecular weight of each?
  Standard 1: Molecular weight 50,000 g/mol
  Standard 2: Molecular weight 40,000 g/mol
  Standard 3: Molecular weight 35,000 g/mol
  Standard 4: Molecular weight 20,000 g/mol
  Standard 5: Molecular weight 10,000 g/mol
  Unknown A: Peak amount polymer eluted at 7.4 min
  Unknown B: Peak amount polymer eluted at 8.4 min
  Unknown C: Peak amount polymer eluted at 9.6 min
 
 

  b. A problem commonly encountered in SEC is overloading the chromatography column by
  injection of a highly concentrated sample, which results in broadening of the peak on the
  chromatogram. You injected a highly concentrated sample of unknown D into the
  chromatography column. Which of the following curves would result? Pick X or Y and
  describe why, based on molecular interactions of the sample with the column.
 

 
 
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