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Home Q & A Board Biology-Related Homework Help Cell Biology
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alatq33 alatq33
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13 years ago
The following shows a restriction endonucleases cleave DNA. abbrev R represents the purines (A&G) and pyrimidines (C, T & U) are abbrev Y. The abbrev W represents A or T.

Enzyme: PpuMI
Targe Sequence: 5' RGGWCCY 3'
                            3' YCCWGGR 5'

Cleavage: 5'RG           GWCCY 3'
                 3' YCCWG          GR 5'

Question: Plasmid DNA and a gene of interest are cut with the enzyme PpuMI. Write a possible sequence of bases for the sticky end of the gene in the 5' to 3' direction.
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wrote...
#1
Educator
13 years ago
Hi alatq33, thos elook like palindromic sequences right?

Just out of curiosity, what textbook are you using?


5' - RG
        GWCCY - 3'

       GWCCY - 3'
5'- RG

Pseudomonas putida is the genomic source for PpuMI
alatq33 Author
wrote...
#2
13 years ago
Bio_man, what is the sticky part of it though?
wrote...
#3
Educator
13 years ago
...RGGWCCY...
...YCCWGGR...

...GWC
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