× Didn't find what you were looking for? Ask a question
Top Posters
Since Sunday
g
3
3
2
J
2
p
2
m
2
h
2
s
2
r
2
d
2
l
2
a
2
Home Q & A Board Biology-Related Homework Help Genetics and Developmental Biology
New Topic  
ppk ppk
wrote...
Valued Member
On Hiatus
Posts: 3561
Rep: 58 2
13 years ago
Mammalian ES cells grown in culture on a “feeder” layer of fibroblasts can be made to differentiate into several types of differentiated cells by treatment with appropriate growth factors, but the feeder layer is essential.  What are two possible functions of the fibroblast layer, and why should it be necessary?
Read 1008 times
1 Reply

Related Topics

Replies
wrote...
#1
Educator
13 years ago
Here are the possibilities:

1) there may be required growth factors unknown to the experimenters that are supplied by the fibroblasts.

2) The fibroblasts may provide an ECM like substrate which supplies signaling co-factors or other cell-surface components required for differentiation.

3) The fibroblasts may be necessary to maintain the ES cells in a totipotent state until the signals to differentiate are given. 
New Topic      
Quick Reply
Explore
Post your homework questions and get free online help from our incredible volunteers
  813 People Browsing
 113 Signed Up Today
Start New Topic Take the Tour CoursesNew Study Tools Topics Trending Browse by Textbook
Live Chat
Related Images
  
 15
  
 5860
  
 3960
Your Opinion
Who will win the 2024 president election?
Votes: 3
Closes: November 4
Previous poll results: Who will win the 2024 president election?