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WadeChl WadeChl
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Posts: 705
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6 years ago
How can the recircularization of a restriction-digested vector during a ligation reaction be minimized?
 
  What will be an ideal response?
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wrote...
6 years ago
The restriction-digested vector can be treated with alkaline phosphatase to remove the 5' phosphates without which the DNA ligase is unable to form a phosphodiester bond.
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