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meli3198 meli3198
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6 years ago
The restriction enzyme SmaI cuts DNA at the 6-base recognition sequence 5'-CCCGGG-3'. Calculate the frequency of occurrence of this sequence. (Assume a 50 GC content.)
 
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wrote...
6 years ago
Assuming a 1:1 AT:CG ratio in the genome and a random distribution of nucleotides, each nucleotide pair will occur with equal frequency ( ). The 6-nucleotide sequence would then occur with a frequency of ( )6, 0.00024, cutting once in every 4,096 base pairs.
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