In this figure showing SDS-PAGE of the progressive stages of glycine N-methyltransferase purification, the 14,000 MW protein most likely disappears from the sample because gel-exclusion chromatography differentiates proteins by:
A. charge-to-mass ratio.
B. mass.
C. charge.
D. solubility.
E. specific affinity to resin.
Accelerated evolution has occurred in all of the following types of human genes except those for:
A. chemosensory perception.
B. immune function.
C. reproduction.
D. transcription factors.
E. brain development or size.
In this figure showing SDS-PAGE of the progressive stages of glycine N-methyltransferase purification, the marker lane indicates that the purified protein:
A. has approximately 32,000 amino acids.
B. has a molecular mass of approximately 32,000.
C. has a positive charge and a molecular mass of approximately 32,000.
D. has a negative charge and a molecular mass of approximately 32,000.
E. is only present when cells are induced with glycine.
An allosteric enzyme most clearly displays positive cooperativity when:
A. Km= K0.5.
B. an increase in substrate increases the rate of reaction.
C. a decrease in substrate concentration is observed soon after initiating the reaction.
D. a minimal increase in modulator is associated with a large increase in V0.
E. a minimal decrease in modulator is associated with a large increase in V0.
Pol III holoenzyme of E. coli is composed of:
A. three Pol III cores, two clamps, two clamp loaders.
B. two Pol III cores, two clamps, and one clamp loader.
C. two Pol III cores, two clamps, and two clamp loaders.
D. three Pol III cores, two clamps, one clamp loader.
E. three Pol III cores, three clamps, one clamp loader.
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