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Home Q & A Board Biology-Related Homework Help General Biology
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tsing_lee tsing_lee
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2 years ago
Hello, could you please tell me what are the differences between RNA seq and RAD seq?
How they work?
Thank you
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bio_manbio_man
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2 years ago
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tsing_lee Author
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2 years ago Edited: 2 years ago, tsing_lee
Thank you so much!
Post Merge: 2 years ago

The, what is the differentiation between RAD seq and RNA seq?
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2 years ago Edited: 2 years ago, bio_man
RNA-Seq is a sequencing technique which uses next-generation sequencing (NGS) to reveal the presence and quantity of RNA in a biological sample at a given moment, analyzing the continuously changing cellular transcriptome.

RNA-Seq does not have an upper limit for quantification, which correlates with the number of sequences obtained. Consequently, it has a large dynamic range of expression levels over which transcripts can be detected.

RNA-Seq has also been shown to be highly accurate for quantifying expression levels, as determined using quantitative PCR (qPCR) and spike-in RNA controls of known concentration. The results of RNA-Seq also show high levels of reproducibility, for both technical and biological replicates. Finally, because there are no cloning steps, and no amplification step, RNA-Seq requires less RNA sample.
Source  https://en.wikipedia.org/wiki/RNA-Seq
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