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2 months ago
Use the following fragment of a polypeptide sequence to perform "reverse translation" to determine the mRNA sequence.
V Asp Trp Leu Lys Gly Asn Thr

 a. Compare your mRNA sequence to the sequence of at least two of your lab mates. Are they the same? Can you use more than one codon for each amino acid?
b. Perform reverse transcription using your mRNA sequence to determine the DNA from which it was transcribed?
c. Explain why the flow of genetic information could not be polypeptide RNA->DNA?
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2 months ago
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V Asp Trp Leu Lys Gly Asn Thr

What's with the "V" at the beginning. Is that short for valine?

I'm going to assume it's a typo and only focus on Asp Trp Leu Lys Gly Asn Thr

What's interesting is that due to the wobble hypothesis, each amino acid can have multiple codons.

(a) That said, your code and your classmates may have different sequences of nucleotides that depict the mRNA. That doesn't mean you are wrong, or they are wrong, as multiple codes do translate to the same amino acid.

AspTrpLeuLysGlyAsnThr
GAU, GACUGGUUA, UUG, CUU, CUC CUA, CUGAAA, AAGGGU, GGC, GGA, GGGAAU, AACACU, ACC, ACA, ACG

Here are all the possible codons to generate, so select one from each column. I've randomly selected those underlined.

GAC-UUG-AAG-GGU-AAU-ACG

(b) Lets convert this back to DNA:

GAC-UUG-AAG-GGU-AAU-ACG
CTG-AAC-TTC-CCA-TTA-TGC

Quote
c. Explain why the flow of genetic information could not be polypeptide RNA->DNA?

Because DNA contains the nucleotide thymine, in mRNA that's replaced with Uracil, among many other reasons.
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