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Jane Doe Jane Doe
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4 years ago
34.  Why?
Select one:
a. It must use different restriction endonucleases because the bacterial and foreign DNA sequences are exactly the same.
b. It must use same restriction endonuclease so that the restriction sites are identical in both foreign and bacterial DNA.
c. It must use same restriction endonuclease so that the restriction sites are different in both foreign and bacterial DNA.
d. It must use different restriction endonucleases because the bacterial and foreign DNA sequences are different.

DNA fingerprinting uses a process called gel electrophoresis to separate the fragments of DNA. Once the DNA fragments are sorted, the pattern of bands can be analyzed.

Gel Electrophoresis Procedure


The smaller DNA fragments start to move away from the wells and the larger DNA fragments remain closer to the wells.
An electric current is passed through the gel.
DNA fragments are treated with a dye.
A restriction endonuclease is added to the DNA.
Using micropipettes, the DNA samples are added to the wells.
DNA fingerprint is produced.
DNA fragments are produced.

The order in which a DNA fingerprint is produced using gel electrophoresis is Answer, Answer, Answer, Answer, Answer, Answer, and Answer.
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2 Replies

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Replies
wrote...
Educator
4 years ago
First question is incomplete. The question is "Why?" Why what?

For the second question, review this topic: https://biology-forums.com/index.php?topic=1822768.0
Jane D. Author
wrote...
4 years ago
The question why refers back to questions 32,33,34. I have the answers to 32 and 33.

32. What is a restriction endonuclease?
Select one:
a. It restricts the movement of the DNA outside the nucleus.
b. It is an enzyme that separates the DNA double helix.
c. It is an enzyme that cleaves at a specific nucleotide sequence.
d. It proofreads the DNA for accidental damages and corrects any errors.


33.The same restriction endonuclease must be used to excise the foreign DNA and bacterial DNA.
Select one:
True
False

34.Why?
Select one:
a. It must use different restriction endonucleases because the bacterial and foreign DNA sequences are exactly the same.
b. It must use same restriction endonuclease so that the restriction sites are identical in both foreign and bacterial DNA.
c. It must use same restriction endonuclease so that the restriction sites are different in both foreign and bacterial DNA.
d. It must use different restriction endonucleases because the bacterial and foreign DNA sequences are different.
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