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In the whole-genome shotgun method, the genome is fragmented and individual fragments are sequenced. ...

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In the whole-genome shotgun method, the genome is fragmented and individual fragments are sequenced. ...

In the whole-genome shotgun method, the genome is fragmented and individual fragments are sequenced. How do we determine the order of the nucleotides in the intact chromosome?

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The fragmentation is done in a very systematic way such that the physical arrangement of fragments is readily apparent.

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The sequences of the ends of the fragments overlap with the ends of other fragments.

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We supplement our information with data from a different technique to determine the final chromosome arrangement.

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DNA hybridization assays are conducted to determine the physical arrangement of the genes on the chromosome.

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Biology: Exploring the Diversity of Life

Edition: 4^th

Authors: Russell, Hertz, McMillan, Fenton

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