Genotype: MATa Δura3 HIS3 LEU2 TRP1 MAL2-8c SUC2 XII-5:: PcPsiM-PcPsiK XI-3:: CrTdc-PcPsiH XI-1:: PcCpr ric1:: X-4:: ARO1-ARO2 XII-4:: ARO4K229LTRP2S65R,S76L Ty-4:: PcPsiM
You using this article? Each of those terms tells you have the genes the strain has. For example, information on
SUC2 can be found if you follow the link. According to the paper I upload, this strain is
ST9318 -- you can email the author for more information, I've tried it in the past and it works 50% of the time.
So the nomeclarture differs from species to species. I know that delta means "deletion" for certain species of bacteria. Have you seen this Wikipedia article?
https://en.wikipedia.org/wiki/Gene_nomenclature#Species-specific_guidelines I've also uploaded the guideline for baker's yeast
Yup, that's the article I'm using and trying to replicate.
The strain that had the highest titer was
ST9482 and it's parent strain was
ST9318 , unless I'm misreading the result?
The nomenclature attachment and links are super helpful, thanks for that.
I guess my current outstanding questions that I'm trying to work out are:
* What base strain of yeast can be used to replicate the transformation? (and where can I get it)
* How does the "parent" strain change the cassette design if at all?
* And generally speaking, since I now have the genotype, how do I reconstruct the entire transformation cassette so that I can get it synthesized and perform the transformation? For example, I need to know which restriction sites and locations were used to insert the genes. Maybe that's in the notation - I'm about to go study those and see.
I did reach out to the authors and we are connected; however, they started a company after publishing the article doing exactly what the paper is doing (producing psilocybin from yeast) and could not offer much due to a conflict of interest concerns.
Building a company that produces psychedelics like psilocybin for clinical treatment of mental health is what I'm exploring and building a working prototype by replicating this paper is my next milestone.