Replicating DNA Cassette from Parent Strain, Plasmid Vector and Genotype

You using this article?

Each of those terms tells you have the genes the strain has. For example, information on SUC2 can be found if you follow the link.

According to the paper I upload, this strain is ST9318 -- you can email the author for more information, I've tried it in the past and it works 50% of the time.

So the nomeclarture differs from species to species. I know that delta means "deletion" for certain species of bacteria. Have you seen this Wikipedia article?

https://en.wikipedia.org/wiki/Gene_nomenclature#Species-specific_guidelines

I've also uploaded the guideline for baker's yeast

Yup, that's the article I'm using and trying to replicate.

The strain that had the highest titer was ST9482 and it's parent strain was ST9318 , unless I'm misreading the result?

The nomenclature attachment and links are super helpful, thanks for that.

I guess my current outstanding questions that I'm trying to work out are:
* What base strain of yeast can be used to replicate the transformation? (and where can I get it)
* How does the "parent" strain change the cassette design if at all?
* And generally speaking, since I now have the genotype, how do I reconstruct the entire transformation cassette so that I can get it synthesized and perform the transformation? For example, I need to know which restriction sites and locations were used to insert the genes. Maybe that's in the notation - I'm about to go study those and see.

I did reach out to the authors and we are connected; however, they started a company after publishing the article doing exactly what the paper is doing (producing psilocybin from yeast) and could not offer much due to a conflict of interest concerns.

Building a company that produces psychedelics like psilocybin for clinical treatment of mental health is what I'm exploring and building a working prototype by replicating this paper is my next milestone.

You sure it doesn't say anything in the materials and methods? You should be able to replicate a study theoretically by what's stated in there

This isn't my field of expertise by take a read





sry I can't offer any more concrete answers to your problem, rly hope you figure it through

TY :)

Thank you