Dilution factor question double check

Mateyman

wrote...

Posts: 142

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3 years ago

Hello all!

Here is my question: If you add 1 μL of PBS buffer into 1000 μL of water, what is the dilution factor?

Isn't dilution factor calculated using this equation: X/X+Y=Z

X = the volume to be transferred to successive tubes

Y = the volume of solution in each tube initially

Z = the dilution factor

So if I plug in

1uL/1uL+1000uL = 9.99x10-4 or 9.99e-4 or 0.0009

OR did I mess this up? Thank you!

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habiba

wrote...

Staff Member

3 years ago

DF = vf / vi

vf = 1 μL + 1000 μL = 1001 μL
vi = 1 μL

1001/1 = 1001

You have diluted the sample by a factor of 1001.

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bio_man

wrote...

Educator

3 years ago

Hi Mateyman

Decided to make a video out of this topic:

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Mateyman Author

wrote...

3 years ago

Thanks for the replies!

So the formula used here:

Is different from the formula used from my class:

Using the first formula I get 1001 uL

Using formula from my class I get 1/1001 = 0.00099999 uL

The 1001 uL makes more sense for sure but I was not sure if the answer using the formula from my class also works maybe?

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bio_man

wrote...

Educator

3 years ago

It's the same formula.

Stock concentration * dilution factor = final concentration

Rearrange, you get:

Dilution factor = final concentration / stock concentration

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