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i_love_science i_love_science
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A month ago
One mechanism involved in apoptosis is the digestion of DNA by DNAases. DNA gets digested into fragments of lengths equal to multiples of the distance between nucleosomes.

The figure attached shows the results of separation by gel electrophoresis of the DNA released by the action of DNAase.

Suggest how the pattern in the left-hand column would change if very high concentrations of DNAase were applied to the cells.

The answer is that there would only be a 250 bp fragment, and it would be brighter.

I think that there would just more fragments of all sizes, as more DNA is degraded. I don't understand why there wouldn't be fragments of larger sizes. Thanks!
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bio_manbio_man
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A month ago
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I believe the answer is larger and brighter is because DNase targets parts of the DNA that is available to be cleaved. Inactive, sequestered genes should remain intact, while active, exposed genes should have been degraded by the enzyme.
Source > https://biology-forums.com/index.php?topic=1564971.0
> https://biology-forums.com/index.php?topic=1564974.0
> https://biology-forums.com/index.php?topic=29371.0
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