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Home Q & A Board Biology-Related Homework Help Biochemistry
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i_love_science i_love_science
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2 years ago
I know that the substrate concentration affects enzyme activity, but does the volume of substrate affect enzyme activity?

For an example situation, beaker 1 has 5 enzymes and 10 substrate molecules in 10 mL. If the volume of substrate is increased in beaker 2, which still has 5 enzymes, but now has 20 substrate molecules in 20 mL (same concentration, x2 volume), does the enzyme activity change?

Thanks!
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#1
2 years ago
I'd assume so. Specific enzyme activity (usually stated simply as 'specific activity') is the number of enzyme units per ml divided by the concentration of substrate/protein in mg/ml. Specific activity values are therefore quoted as units/mg or nmol/min/mg (if unit definition B is applied).
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#2
A year ago
Enzymes will work best if there is plenty of substrate. As the concentration of the substrate increases, so does the rate of enzyme activity.
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