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Ayushi Ayushi
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2 years ago
 You have designed a 15 base-pair long degenerate primer (sequence: CATCATCATMAAGCM). The Ms represent base pairs which can either be A or C. You will be using these primers for binding within a genome of 2*10^9 base pairs. What is the expected number of binding sites for your primers?
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Staff Member
2 years ago
Seven
- Master of Science in Biology
- Bachelor of Science
Ayushi Author
wrote...
2 years ago
Please explain the answer sir/mam _/\_
Slight Smile  https://www.chemtopper.com/biology.php
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Educator
2 years ago Edited: 2 years ago, bio_man
I believe here's how to approach the problem

First, ask yourself, how many ways can you select the first nucleotides: 4

Again, how many ways can you select the second: 4

So far, 4 \(\times\) 4 (to understand why we multiply, refer to the fundamental counting principle in mathematics -- video for reference provided below).

In fact, that's the case for the first 9 nucleotides, so \(4\cdot 4\cdot 4\cdot 4\cdot 4\cdot 4\cdot 4\cdot 4\cdot 4=4^9\).

The tenth nucleotide is an M. From the condition provided in the question, there are only 2 ways you can select for M, either A or C.

The next 4 nucleotides, again, are all 4, therefore 44, and the last can be selected in 2 ways again since it is an M.

\(4^9 \cdot 2 \cdot 4^4 \cdot 2 = 4^{14}\)

\((2 \times 10^9) ÷ 4^{14} \approx 7\)
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