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V. Villanueva V. Villanueva
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When a DNA mismatch is detected in E.coli, what mechanism allows the cell to determine which was more likely to be the original nucleotide?
Textbook 

Genetic Analysis: An Integrated Approach


Edition: 3rd
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FreddyK10FreddyK10
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The identification is accomplished by the sensitivity of mismatch repair enzymes to the methylation (the addition of CH3 groups) of specific nucleotides in the original DNA strand. In E. coli, methylation is a common feature of DNA, and prior to replication, DNA parental strands are fully methylated. However, the daughter DNA duplexes produced by replication are only hemimethylated- that is, fully methylated on just the parental strand. A period of time must pass before the newly synthesized strands are fully methylated, and it is in this window of time that the methyl-sensitive components of mismatch repair operate.

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