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Jappyde1 Jappyde1
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A gene, VTE4, from the sunflower of known sequence and well-understood transcriptional regulation, is available from another lab. The product of this gene enables sunflowers to to produce a significantly increased amount of vitamin E from a substrate produced in all plants.  The plant Brassica napus is harvested for canola oil. To produce canola oil with increased vitamin E, how would you proceed?

▸ Select Brassica plants with the highest titer of the vitamin, isolate the genes responsible, and cause these genes to duplicate.

▸ Use a yeast system to engineer yeasts that produce the vitamin, and get manufacturers to add this to the canola oil during bottling.

▸ Introduce the gene and necessary cis elements into Brassica, along with a reporter gene, then measure the vitamin E produced.

▸ Clone VTE4 in A. tumefaciens and use these bacteria to produce transgenic Brassica.

▸ Introduce VTE4 into tomato plants or another food that humans eat more than Brassica.
Textbook 

Genetic Analysis: An Integrated Approach


Edition: 3rd
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loki1345loki1345
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Introduce the gene and necessary cis elements into Brassica, along with a reporter gene, then measure the vitamin E produced.

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