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khayes1 khayes1
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3 weeks ago
Efficient production of eukaryotic proteins in E. coli may require all of the following except?

▸ an E. coli expression vector with a promoter sequence that binds RNA polymerase

▸ an E. coli expression vector with a Shine-Dalgarno sequence for efficient translation

▸ altered codon usage within the heterologous sequences to approximate the codon bias in E. coli

▸ the use of cDNAs, that are free of introns, as eukaryotic transgenes

▸ a negative selectable marker to select against nonhomologous recombination events
Textbook 

Genetic Analysis: An Integrated Approach


Edition: 3rd
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BoogyBoogy
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a negative selectable marker to select against nonhomologous recombination events

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