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If bacteria transformed with a recombinant pUC18 plasmid produce white colonies when grown on plates ...

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If bacteria transformed with a recombinant pUC18 plasmid produce white colonies when grown on plates ...

If bacteria transformed with a recombinant pUC18 plasmid produce white colonies when grown on plates containing ampicillin and Xgal, which of the following is least likely?

▸ They produce β-lactamase that provides resistance to ampicillin.

▸ They produce functional β-galactosidase that cleaves X-gal.

▸ They carry a vector with the bla gene used as the selectable marker.

▸ They carry a vector that contains a DNA fragment inserted into the multiple cloning site.

▸ They carry a vector with a lacZ gene that has been disrupted and rendered nonfunctional.

Textbook

Genetic Analysis: An Integrated Approach

Edition: 3^rd

Authors: Sanders, Bowman

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