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vanessa_vela vanessa_vela
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Which of the following lists the steps for cDNA library preparation in the correct order?

I.Partially degrade the mRNA with RNase H
II.Isolate mRNA from a specific cell/ tissue with an oligo-dT primer that hybridizes to poly-A tail
III.Synthesize a strand of cDNA using DNA polymerase with mRNA fragments as primers
IV.Ligate cDNAs into a cloning vector
V.Create a single-stranded DNA molecule complementary to the mRNA using reverse 
transcriptase


▸ I, II, III, IV, V

▸ II, V, I, III, IV

▸ II, III, V, I, IV

▸ V, III, I, IV, II

▸ II, V, IV, III, I
Textbook 

Genetic Analysis: An Integrated Approach


Edition: 3rd
Authors:
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festuca96festuca96
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II, V, I, III, IV

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