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You have isolated a plasmid fromE. coli. You get the following results, using three restriction ...

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You have isolated a plasmid fromE. coli. You get the following results, using three restriction ...

You have isolated a plasmid from E. coli. You get the following results, using three restriction enzymes, 1 XhoI, 2 BamHI, and 3 EcoRI to perform six different digestions: single digests using each enzyme alone and double digests using each combination of two enzymes.

enzyme	# fragments	sizes (in kb)
XhoI	1	10
BamHI	2	3.75, 6.25
EcoRI	2	4.25, 5.75
XhoI + BamHI	3	1.25, 3.75, 5
XhoI + EcoRI	3	2, 3.75, 4.25
BamHI + EcoRI	4	1.25, 2.5, 3, 3.25

Draw a restriction map of the plasmid.

Textbook

Genetic Analysis: An Integrated Approach

Edition: 3^rd

Authors: Sanders, Bowman

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