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Izzy76 Izzy76
wrote...
Posts: 2
Rep: 0 0
A month ago
Hey there,
I am currently working on constructing a plasmid used for biosynthetic cocaine and want some reviews on it to spot some mistakes i may have made. I overexpressed all off the precursors and added a histidine tag for purification. All the genes are from uniprot.
Also i want to know which genes to knockout to improve Flux.
Here is the Link https: //benchling.com/s/seq-tW7Olg2xpVhawgY4FqyI?m=slm-7auYvm6cfA9merXCuLL1

Help much appreciated
Izzy
Read 113 times
2 Replies

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Replies
wrote...
Educator
A month ago
Hi Izzy76

You lost me at "plasmid used for biosynthetic cocaine". You're planning on inserting this plasmid into a species to produce synthetic cocaine. Is this a novel procedure that you invented? Where can I read up on it?
Izzy76 Author
wrote...
A month ago
It is a novel procedure i cam up with. Did some research about similiar molecules like tropine and made this plasmid as result
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