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Home Q & A Board Biology-Related Homework Help Genetics and Developmental Biology
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Anonymous Thao vy Tran
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2 weeks ago
How to know about the number of yeast's chromosome? Is there any method to know aout that ?
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#1
Educator
2 weeks ago
Hello

I believe the most basic way is to first isolate the DNA using any known technique such as phenol-chloroform extraction (linked below). Once the DNA of the species has been isolated, the DNA is stained and the chromosomes can be visualized under a microscope using staining techniques, such as Giemsa staining or fluorescent dyes that bind to DNA. During cell division (specifically, metaphase), chromosomes condense and become visible.

Link to phenol-chloroform extraction kit: https://www.thermofisher.com/ca/en/home/references/protocols/nucleic-acid-purification-and-analysis/dna-extraction-protocols/phenol-chloroform-extraction.html

Hope that helps!
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