× Didn't find what you were looking for? Ask a question
Top Posters
Since Sunday
g
3
3
2
J
2
p
2
m
2
h
2
s
2
r
2
d
2
l
2
a
2
New Topic  
Gardenia Gardenia
wrote...
Posts: 2
Rep: 0 0
9 years ago
I am talking about the standard DNA isolation experiment from kiwis and onions.Protocol goes something like this (general and conceptual version follows)

1) Chop the kiwi/onion up finely and add it to a solution of NaCl and detergent
2)Incubate at high temp for a bit
3)Now incubate at cold temp
4)mix well and filter
5)Add the extract to a boiling tube and add neutrase

Here's the bit I need explained

6)Slowly trickle x amount of ethanol VERY SLOWLY down the side of the test tube

Why do you add it slowly and why do you not add it directly to the solution?
Read 2139 times
1 Reply

Related Topics

Replies
wrote...
Educator
9 years ago Edited: 9 years ago, bio_man
Why do you add it slowly and why do you not add it directly to the solution?

You want a single layer of clear ethanol on top of the mixture. COLD ethanol will work best, as it's the best practice for precipitation.
New Topic      
Explore
Post your homework questions and get free online help from our incredible volunteers
  1055 People Browsing
 115 Signed Up Today
Related Images
  
 672
  
 206
  
 1017
Your Opinion
Who will win the 2024 president election?
Votes: 3
Closes: November 4