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Gardenia Gardenia
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9 years ago
I am talking about the standard DNA isolation experiment from kiwis and onions.Protocol goes something like this (general and conceptual version follows)

1) Chop the kiwi/onion up finely and add it to a solution of NaCl and detergent
2)Incubate at high temp for a bit
3)Now incubate at cold temp
4)mix well and filter
5)Add the extract to a boiling tube and add neutrase

Here's the bit I need explained

6)Slowly trickle x amount of ethanol VERY SLOWLY down the side of the test tube

Why do you add it slowly and why do you not add it directly to the solution?
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Valued Member
Educator
9 years ago Edited: 9 years ago, bio_man
Why do you add it slowly and why do you not add it directly to the solution?

You want a single layer of clear ethanol on top of the mixture. COLD ethanol will work best, as it's the best practice for precipitation.
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