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Home Q & A Board Biology-Related Homework Help Genetics and Developmental Biology
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pisker pisker
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8 years ago
Hello, I'm new here so sorry if this is not the appropriate way to ask, but for a school project, we recently sequenced DNA that codes for the production of an enzyme in a plant that has not had this specific gene sequenced yet. What I need is help to find the locations of the introns and the exons. Is there a program that I can use to do this? Thanks!
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#1
Educator
8 years ago
This is being done at the high school level?

Have you been told the chromosome position or what the gene is?
pisker Author
wrote...
#2
8 years ago
Yes it is.

http://www.genecards.org/cgi-bin/carddisp.pl?gene=GAPDH

The gene is GAPDH, but I am unsure of the chromosomal position.
wrote...
#3
Educator
8 years ago Edited: 8 years ago, bio_man
You can get the entire gene sequence or the mRNA sequence from NCBI. NCBI also has exon and intron information but may be a little confusing. You can paste the mRNA sequence (if you happen to have it) in the program called BLAT (https://genome.ucsc.edu/cgi-bin/hgBlat?command=start) on UCSC genome browser.

Have you tried that?
pisker Author
wrote...
#4
8 years ago
Hmm... Every site seems to be giving me slightly different results... I'm not sure what I'm doing incorrectly...
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