DNA fingerprinting probes

ehd123

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8 years ago

DNA fingerprinting probes

What is the nature and some properties of probes used in DNA fingerprinting?

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B.Sc in Biology
M.Sc Neuroscience
PhD. Candidate in Neuroscience

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bio_man

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8 years ago

I believe their are single-locus and multi-locus probes.

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ehd123 Author

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8 years ago

I don't even know what these mean..
I was thinking along the lines of short, specific, complementary, single stranded DNA sequence, labelled... What do you think?

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B.Sc in Biology
M.Sc Neuroscience
PhD. Candidate in Neuroscience

bio_man

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Educator

8 years ago

I got my idea from this website: http://www.biology.arizona.edu/human_bio/problem_sets/dna_forensics_2/04t.html

A single locus probe is a DNA or RNA sequence that is able to hybridize (i.e. form a DNA-DNA or DNA-RNA duplex) with DNA from a specific restriction fragment on the Southern blot. Duplex formation depends on complementary base pairing between the DNA on the Southern blot and the probe sequence. Single locus probes are usually tagged with a radioactive label for easy detection, and are chosen to detect one polymorphic genetic locus on a single human chromosome. The Southern blot from step 4 is incubated in a solution containing a radioactive, single locus probe under conditions of temperature and salt concentration that favor hybridization. After hybridization, the unbound probe is washed away, so that the only radioactivity remaining bound to the nylon membrane is associated with the DNA of the targeted locus.

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ehd123 Author

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8 years ago

The idea is similar but the wording is fancy lol
I think single locus probes means specific to a sequence and not binding everywhere which is the point of DNA fingerprinting
Thank you very much Upwards Arrow

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B.Sc in Biology
M.Sc Neuroscience
PhD. Candidate in Neuroscience

bio_man

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Educator

8 years ago

Quote from: ehd123 (8 years ago)

I think single locus probes means specific to a sequence and not binding everywhere which is the point of DNA fingerprinting

Maybe that's the multi-probe locus version of it? ... You're welcome

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