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confused_student confused_student
wrote...
13 years ago
Using this data could you help me figure out if these genes are linked and if they are what’s the distance between them? 
This is a dihybrid cross working with fruit flies.  Parental generation is a sepia eyed male x apterous female.  The F1 are all wild type.  The F2 are as follows:
637 +;+
175 +;se
63 ap;+
17 ap;se
Overall F2 ratio observed: 37 wild type: 10 sepia: 3 apterous: 1 apterous sepia
Thank you for any help!!!
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wrote...
Valued Member
13 years ago
9:3:4 due to epistasis.
wrote...
13 years ago
yeehaw!  Muchas Gracias! Smiling Face with Open Mouth
wrote...
13 years ago Edited: 13 years ago, confused_student
If genes are epistatic, this is a kind of linkage right?  The ratio obtained was 37:10:3:1, and this is similar to the 12:3:1 ratio?

I made a linkage map please check this for me and see if I'm right.


F2 progeny is
    
637 +; +
175 +; se
63 ap; +
17 ap; se
 
Recombinants between wild and sepia are 175
Recombinants between sepia and apterous are 17
Recombinants between wild and apterous are 63
Total progeny are 892
 
Frequency of recombination between wild and sepia are 175/892 = 0.196
Frequency of recombination between sepia and apterous is 17 / 892 = 0.323
Frequency of recombination between wild and apterous is 63 / 892 = 0.070
 
The genes are linked and the distance between the genes is
 
Sepia-----------------wild--------apterous
ß------0.196------àß---0.070----à
ß----------------0.323----------------à
 
Overall F2 ratio observed:
37 wild type: 10 wild and sepia: 3 wild and apterous: 1 apterous and sepia
According to chisquare analysis the ratio doesn’t fit the 9:3:3:1 ratio.
wrote...
Educator
13 years ago
Hey confused_student, I downloaded an excellent example for you from one of our paid database. It will describe how it's done. Tell me if it is any help to you! The explanation is on page 1, example is on page 2.
 Attached file 
(129.36 KB)
You must login or register to gain access to this attachment.
wrote...
13 years ago
Thanks bio man!  I'll try to download it but I'm on a dial-up connection, so I may have to wait until tomorrow. 
I'm mainly wondering if it is possible to DO a linkage map for epistatic genes.  Of course, I don't know for sure that these genes are epistatic.  These are my lab results, it's not from the text book.  I realize I posted in the wrong spot.
If a map can be made, I'm reasonably sure I made a good map. 
wrote...
13 years ago
this is all wrong.  I'm freaking out.  please, someone help me!
wrote...
Educator
13 years ago Edited: 13 years ago, bio_man
Don't freak out confused_student.

Clearly then if the chi-square analysis doesn't fit the 9:3:3:1 ratio, then it is epistasis like lewis suggested.

Try to download that file I uploaded, it's really small so I wouldn't really effect anything. The document states:

 Attached file 
Thumbnail(s):
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wrote...
Educator
13 years ago
A common observation in genetics is that the product of several different genes can interact to produce certain phenotypes. Two independent genes (e.g. non-interacting), each with a dominant/recessive pair of alleles, will produce four phenotypes in a 9:3:3:1 ratio in the F2 generation of a dihybrid cross. However, if there is an interaction between the two genes (epistasis) a modified F2 dihybrid ratio may be observed.

A modified F2 dihybrid ratio of 9:3:4 is indicative of recessive epistasis.
wrote...
13 years ago
Okay, I see the 9:3:4 listed as one of the common epistatic ratios.  But in this lab we got 37:10:3:1.  The lab TA cooked the books so that we would come to a conclusion. There is another epistasis ratio 12:3:1, and that's similiar to the last three numbers that the TA gave us.

My hair is on fire!!!!  If it is epistasis, should I try to map it? 
wrote...
Educator
13 years ago
You're left with no other choice. Yes.
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