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Octoshark Octoshark
wrote...
13 years ago
A single base substitution in the anticodon loop of one of the tRNAs for glycine (tRNAgly) can hypothetically generate a mutant tRNA that is able to suppress translational termination by causing insertion of a glycine residue at certain termination codons.  Mutations of this sort are rarely encountered in nature, due in part to the existence of a key step in the chain of events that helps ensure translational fidelity.  What is this step and how might it prevent suppressor tRNAs from being able to incorporate amino acids at stop codons?
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wrote...
Donated
Valued Member
13 years ago
I found an ancient document you may be interested in Undecided... where's bio_man when you need him!
 Attached file 
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wrote...
Educator
13 years ago
Peptide chain release factors prevent suppressor tRNA from being able to incorporate amino acids at the stop codon. The tRNA can directly compete with the termination factor for stop codon binding in the A site much more effectively, resulting in signifant stop codon suppression. In mammalian cells, this appraoch has been shown to supprewss premature  stop mutations that cause certain illnesses like duchenne mucular dystrophy.
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