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trixter47 trixter47
wrote...
13 years ago
A single base substitution in the anticodon loop of one of the tRNAs for glycine (tRNAgly) can hypothetically generate a mutant tRNA that is able to suppress translational termination by causing insertion of a glycine residue at certain termination codons.  Such mutant tRNAs are in fact encountered in nature and are referred to as suppressor tRNAs.  Assume that there are multiple tRNAs for glycine (so that this hypothetical mutation would not be lethal) and that one of these tRNAs acquires a single base mutation in its anticodon loop.


A. What is the nucleotide sequence of the wildtype anticodon of tRNAgly (5’-3’ polarity) and what is the mutant sequence that would have the properties described above?
   
B)  At which stop codon(s) would the mutant tRNAgly be able to suppress translational termination? 
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Replies
wrote...
Educator
13 years ago
Codons of glycine are:

GGU
GGC
5'-GGA-3'
GGG

tRNA anticodon that respond to glycine on the mRNA:

CCA
CCG
3'-CCU-5'
CCC

Some stop codons:

UAA
UAG
UGA

tRNA anticodons that respond to stop codons on the mRNA:

AUU
AUC
ACU

So, to cause insertion of a glycine residue at certain termination codon, the tRNA anticodon for glycine 3'-CCU-5' has to change to 3'-ACU-5', because it is only with an anticodon 3'-ACU-5' that it will substitue a glycine where we have 5'-UGA-3' stop-codon on the mRNA. There are no other possibilities.

You just have to think backwards.
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